| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kleinman, Isabel E (9)
|
MAWAVE |
41.66 |
38.36 |
-3.30 |
| 2 |
White, Addisyn A (10)
|
MANRG |
42.47 |
38.84 |
-3.63 |
| 3 |
Cowan, Kelcie Q (10)
|
MAFSA |
42.39 |
40.38 |
-2.01 |
| 4 |
McAllister, Samira E (10)
|
MANRG |
51.30 |
41.58 |
-9.72 |
| 5 |
Jacob, Emily C (9)
|
MAWAVE |
40.30 |
42.12 |
1.82 |
| 6 |
Jacobson, Raegan P (9)
|
MAWAVE |
|
44.45 |
- |
| 7 |
Ethiraj, Sanjita (10)
|
MAT85 |
48.64 |
45.44 |
-3.20 |
| 8 |
Mantini, Elena B (9)
|
MAFSA |
48.31 |
46.14 |
-2.17 |
| 9 |
Karpinski, Genevieve E (10)
|
MAFSA |
47.94 |
47.25 |
-0.69 |
| 10 |
Simmons, Carly (7)
|
MAT85 |
45.92 |
47.26 |
1.34 |
| 11 |
Wicker, Anna G (10)
|
MANRG |
46.53 |
47.49 |
0.96 |
| 12 |
Moore, Maddie (9)
|
MAT85 |
|
47.80 |
- |
| 13 |
McAveety, Meghan O (9)
|
MAWAVE |
49.39 |
48.25 |
-1.14 |
| 14 |
Pazdunkiewicz, Lucy R (9)
|
MANRG |
|
48.65 |
- |
| 15 |
Martino, Alyssa (7)
|
MAT85 |
53.48 |
48.94 |
-4.54 |
| 16 |
Smith, Shannon M (9)
|
MANRG |
|
49.68 |
- |
| 17 |
Gibson, Grace A (10)
|
MANRG |
|
49.84 |
- |
| 18 |
Wicker, Emily M (8)
|
MANRG |
|
50.68 |
- |
| 19 |
Mahaniah, Leyli K (8)
|
MAFSA |
52.33 |
50.89 |
-1.44 |
| 20 |
Rothman, Gianna (9)
|
MAUN-B |
54.15 |
51.80 |
-2.35 |
| 21 |
Kline, Mari E (8)
|
MAUN-B |
59.21 |
57.68 |
-1.53 |
| 22 |
Hill, Caroline G (9)
|
MAFSA |
1:04.30 |
59.81 |
-4.49 |
| 23 |
Brady, Lucia M (9)
|
MAFSA |
|
1:03.97 |
- |
|
Maroon, Nancy R (10)
|
MAFSA |
|
DQ |
- |
|
Jeong, Chloe N (9)
|
MAFSA |
|
NS |
- |