| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hattala, Caroline E (11)
|
MAEMAC |
36.93 |
36.90 |
-0.03 |
| 2 |
Harmon, Sophia D (12)
|
MAEMAC |
38.87 |
39.07 |
0.20 |
| 3 |
Molnar, Natalie S (12)
|
MAUN-E |
40.55 |
40.51 |
-0.04 |
| 4 |
Klees, Gloria K (12)
|
MAEMAC |
41.78 |
40.53 |
-1.25 |
| 5 |
Shutkufski, Madison H (12)
|
MAEMAC |
41.75 |
42.03 |
0.28 |
| 6 |
Pappentick, Addison K (12)
|
MAEMAC |
44.39 |
42.12 |
-2.27 |
| 7 |
Smith, Eve G (12)
|
MAEMAC |
49.72 |
43.46 |
-6.26 |
| 8 |
Kucera, Addison S (12)
|
MAEMAC |
46.51 |
44.12 |
-2.39 |
| 9 |
Cort, Julia E (11)
|
MAEMAC |
45.43 |
44.96 |
-0.47 |
| 10 |
Kofroth, Priscilla J (12)
|
MAEMAC |
|
45.40 |
- |
| 11 |
Price, Katryna M (12)
|
MAEMAC |
43.56 |
45.84 |
2.28 |
| 12 |
Karboski, Veronica M (12)
|
MAEMAC |
55.84 |
46.12 |
-9.72 |
| 13 |
Reddy, Druthi A (11)
|
MAEMAC |
47.60 |
47.20 |
-0.40 |
| 14 |
Vezenov, Zoe E (11)
|
MAEMAC |
46.76 |
47.95 |
1.19 |
| 15 |
Pechulis, Emelia C (12)
|
MAEMAC |
51.42 |
48.10 |
-3.32 |
| 16 |
Metzger, Averi B (12)
|
MAEMAC |
1:06.31 |
52.32 |
-13.99 |
| 17 |
Varga, Delaney (11)
|
MAEMAC |
53.25 |
53.97 |
0.72 |
| 18 |
Marsh, Arabella C (11)
|
MAEMAC |
|
56.32 |
- |
|
O'Donnell, Mikayla B (11)
|
MAEMAC |
|
DQ |
- |