| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhou, Andrew (12)
|
CTWYW |
37.05 |
35.37 |
-1.68 |
| 2 |
Moon, Peter (11)
|
MAJW |
41.66 |
37.29 |
-4.37 |
| 3 |
Papanikolaou, Marcus P (12)
|
MAGAAC |
39.35 |
37.77 |
-1.58 |
| 4 |
Skidell, Spencer A (12)
|
CTWYW |
41.43 |
40.94 |
-0.49 |
| 5 |
Wang, Jerry Z (11)
|
MASSC |
|
41.17 |
- |
| 6 |
Stanker, Benjamin M (11)
|
MAGPAC |
41.27 |
42.26 |
0.99 |
| 7 |
Whitney, Colby P (12)
|
MAUN-S |
43.04 |
42.29 |
-0.75 |
| 8 |
Smilevski, Maximilian (12)
|
MASEA |
43.35 |
43.27 |
-0.08 |
| 9 |
Johnson, Jacob R (12)
|
MASSC |
44.06 |
43.32 |
-0.74 |
| 10 |
Dugan, Sean C (12)
|
MAGPAC |
44.96 |
44.38 |
-0.58 |
| 11 |
DeSumma, Trevor T (12)
|
MAJW |
45.24 |
45.78 |
0.54 |
| 12 |
Hong, Timothy D (11)
|
MAPCAC |
45.33 |
46.09 |
0.76 |
| 13 |
Hughes, William J (11)
|
MASEA |
37.91 |
46.89 |
8.98 |
|
Broadbelt, Colin D (12)
|
MAJW |
45.21 |
NS |
- |
|
Moyer, Ryan M (11)
|
MAJW |
43.97 |
NS |
- |