| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pringle, Adalie R (12)
|
MAUN-0 |
|
32.67 |
- |
| 1 |
Albrecht, Chloe A (12)
|
MAUN-0 |
33.29 |
32.98 |
-0.31 |
| 2 |
Carliss, Sophia E (12)
|
MDFAST |
33.66 |
33.27 |
-0.39 |
| 2 |
Mitchell, Simone L (12)
|
MABYNS |
39.61 |
35.41 |
-4.20 |
| 3 |
Yao, Nyah R (11)
|
MARAD |
34.46 |
33.38 |
-1.08 |
| 3 |
Gordy, Julia C (11)
|
MDFAST |
|
35.63 |
- |
| 4 |
Rasmussen, Charlotte B (11)
|
MAFWAC |
34.09 |
33.56 |
-0.53 |
| 4 |
Simmons, Emily K (11)
|
MDBAAC |
|
36.64 |
- |
| 5 |
Fabula, Jillian E (12)
|
MDBAAC |
34.56 |
34.61 |
0.05 |
| 5 |
Wirth, Ella R (12)
|
MDFAST |
41.63 |
36.71 |
-4.92 |
| 6 |
Olszyk, Zoe A (12)
|
MDBAAC |
36.60 |
37.22 |
0.62 |
| 7 |
Parrish, Aurora K (12)
|
MAKA |
37.67 |
37.25 |
-0.42 |
| 8 |
Harshman, Madeline M (11)
|
MDFAST |
37.66 |
37.90 |
0.24 |
| 9 |
Lester - Stranick, Sarah R (11)
|
MABYNS |
|
38.28 |
- |
| 10 |
Ellenbecker, Vivienne N (11)
|
MDBAAC |
38.77 |
40.21 |
1.44 |
| 11 |
Chen, Rayna L (11)
|
MDBAAC |
40.58 |
40.91 |
0.33 |
| 12 |
Gilsinn, Laila N (11)
|
MDFAST |
41.88 |
41.20 |
-0.68 |
| 13 |
Sober, Morgan T (12)
|
MAKA |
|
47.32 |
- |