| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reber, Brody D (10)
|
MALAC |
28.84 |
28.63 |
-0.21 |
| 2 |
Shirey, Jackson E (10)
|
MALAC |
29.76 |
29.58 |
-0.18 |
| 3 |
Lehan, Colin S (10)
|
MAHAC |
35.07 |
35.75 |
0.68 |
| 4 |
Wingert, Erik S (10)
|
MAHAC |
37.59 |
36.44 |
-1.15 |
| 5 |
O'Shea, Ryan M (10)
|
MALAC |
38.23 |
38.42 |
0.19 |
| 6 |
Koppmann, Benjamin A (10)
|
MALAC |
38.91 |
40.01 |
1.10 |
| 7 |
Rush, Gavin M (9)
|
MALAC |
41.38 |
40.31 |
-1.07 |
| 8 |
Brace, Colton (10)
|
MAHAC |
39.25 |
40.89 |
1.64 |
| 9 |
Hulays, Max D (10)
|
MAHAC |
41.20 |
41.79 |
0.59 |
| 10 |
Chetlen, Nathaniel L (10)
|
MAHAC |
40.71 |
41.81 |
1.10 |
| 11 |
Hollerbach, Grant T (9)
|
MAHAC |
44.65 |
41.91 |
-2.74 |
| 12 |
Hollerbach, Jack T (10)
|
MAHAC |
47.95 |
42.10 |
-5.85 |
| 13 |
Moll, Justin C (9)
|
MALAC |
42.62 |
42.80 |
0.18 |
| 14 |
Zhu, Albert Z (10)
|
MAHAC |
45.36 |
44.41 |
-0.95 |
| 15 |
Paul, Ainesh (9)
|
UNHA |
|
47.31 |
- |
| 16 |
LI, Daniel Z (10)
|
MAHAC |
|
52.75 |
- |
| 17 |
Baweja, Ritvik (10)
|
MAHAC |
|
59.20 |
- |