| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burkholder, Cali G (10)
|
MALAC |
31.93 |
31.52 |
-0.41 |
| 2 |
Clark, Maura L (10)
|
MALAC |
33.59 |
34.00 |
0.41 |
| 3 |
Reber, Peyton E (9)
|
MALAC |
34.48 |
35.44 |
0.96 |
| 4 |
Paterno, Veronica S (10)
|
MAHAC |
36.46 |
35.68 |
-0.78 |
| 5 |
Mahler, Madeline J (9)
|
MAHAC |
35.71 |
35.89 |
0.18 |
| 6 |
Planck, Avery N (10)
|
MALAC |
38.58 |
36.04 |
-2.54 |
| 7 |
Kesterson, Avery B (10)
|
MAHAC |
36.60 |
36.81 |
0.21 |
| 8 |
Kowitz, Lucy M (10)
|
MALAC |
34.84 |
37.25 |
2.41 |
| 9 |
Eshleman, Natalie N (10)
|
MALAC |
36.60 |
37.59 |
0.99 |
| 10 |
Scheirer, Hailey N (9)
|
MALAC |
38.98 |
39.98 |
1.00 |
| 11 |
Darowish, Maya J (10)
|
MAHAC |
41.73 |
41.95 |
0.22 |
| 12 |
DiGiovanna, Claire (10)
|
MAHAC |
48.05 |
42.21 |
-5.84 |
| 13 |
Virabyan, Angelika J (10)
|
MAHAC |
41.73 |
43.47 |
1.74 |
| 14 |
Stalin, Isha (9)
|
MAHAC |
|
44.06 |
- |
| 15 |
Borromeo, Tyra M (9)
|
MAHAC |
|
45.23 |
- |
| 16 |
Galbraith, Maya R (9)
|
MALAC |
43.16 |
46.64 |
3.48 |
| 17 |
Karamchandani, Diya (10)
|
MAHAC |
|
47.43 |
- |
| 18 |
Holt, Eleanora G (9)
|
MAHAC |
|
47.81 |
- |
| 19 |
Willoughby, Abiageal E (10)
|
MALAC |
48.37 |
51.07 |
2.70 |
| 20 |
Risser, Addison L (10)
|
MALAC |
48.58 |
51.29 |
2.71 |