| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Hailey E (6)
|
GUCFSC |
19.48 |
19.96 |
0.48 |
| 2 |
Rogojina, Kaitlyn G (6)
|
GUCFSC |
23.62 |
23.97 |
0.35 |
| 3 |
Rosebush, Emilee A (6)
|
GUMAC |
26.58 |
24.08 |
-2.50 |
| 4 |
Betts, Mia K (6)
|
GUCFSC |
27.43 |
24.12 |
-3.31 |
| 5 |
Howard, Piper E (6)
|
GUMARC |
29.38 |
24.18 |
-5.20 |
| 6 |
Hargus, Brenna K (6)
|
GUMAC |
28.47 |
27.16 |
-1.31 |
| 7 |
Galvez, Sabine A (6)
|
GUESCA |
31.05 |
28.44 |
-2.61 |
| 8 |
Kable, Olivia M (5)
|
GUTASC |
32.82 |
30.04 |
-2.78 |
| 9 |
Gaitan, Helena L (5)
|
GUCFSC |
42.95 |
39.04 |
-3.91 |
|
McCarthy, Gabrielle R (6)
|
GUCFSC |
|
NS |
- |
|
Nguyen, Tianna K (6)
|
GUESCA |
27.57 |
NS |
- |
|
Kiyashchenko, Darya (5)
|
GULIFE |
26.40 |
NS |
- |