| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Delarosa, Joseph Christopher (12)
|
NTMAC |
43.80 |
38.32 |
-5.48 |
| 2 |
Souter, Emmanuel J (12)
|
NTMAC |
40.53 |
39.17 |
-1.36 |
| 3 |
Premmanee, Kuhnnachupb (11)
|
NTMAC |
43.84 |
41.22 |
-2.62 |
| 4 |
Park, Jonah (11)
|
NTMARS |
41.88 |
41.52 |
-0.36 |
| 5 |
Nguyen, Avery N (11)
|
NTLAC |
42.06 |
42.01 |
-0.05 |
| 6 |
Collins, Boaz Y (11)
|
NTMAC |
44.46 |
42.54 |
-1.92 |
| 7 |
Werth, Nolan M (11)
|
NTUN |
52.65 |
43.67 |
-8.98 |
| 8 |
Alferink, Kyle G (11)
|
NTUN-M |
47.28 |
44.15 |
-3.13 |
| 9 |
Hamer, Abi D (11)
|
NTMTRO |
|
45.08 |
- |
| 10 |
Traub, Gabriel M (12)
|
NTLAC |
46.56 |
45.37 |
-1.19 |
| 11 |
Starnes, Drew M (12)
|
NTLAC |
47.27 |
46.25 |
-1.02 |
| 12 |
Heron, Benjamin T (12)
|
NTLAC |
46.16 |
46.75 |
0.59 |
| 13 |
Moon, Andrew J (11)
|
NTMARS |
57.56 |
47.98 |
-9.58 |
| 14 |
Thomas, London X (11)
|
NTMTRO |
53.21 |
50.57 |
-2.64 |
| 15 |
Tatari, Tyler A (11)
|
NTLAC |
51.53 |
50.59 |
-0.94 |
| 16 |
Olsen, Christian E (11)
|
NTLAC |
52.61 |
51.40 |
-1.21 |
| 17 |
Camargo Chavez, Cesar (11)
|
NTMTRO |
56.33 |
51.52 |
-4.81 |
| 18 |
Sieg, Karl C (11)
|
NTLAC |
56.07 |
52.11 |
-3.96 |
| 19 |
Camargo Chavez, Antonio (12)
|
NTMTRO |
52.46 |
52.33 |
-0.13 |
| 20 |
Akula Kamalesh, Trinay (12)
|
NTLAC |
51.46 |
52.75 |
1.29 |
| 21 |
Greiner, Mason M (12)
|
NTLAC |
58.15 |
54.47 |
-3.68 |
| 22 |
Willert, Matthew G (11)
|
NTMTRO |
56.91 |
55.68 |
-1.23 |
| 23 |
Hulme, Mitchell S (11)
|
NTMARS |
59.52 |
58.47 |
-1.05 |
|
Tatari, Evan S (11)
|
NTLAC |
|
DQ |
- |