| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Beck, Jackson E (11)
|
NTFAST |
59.50 |
56.53 |
-2.97 |
| 2 |
Go, Hayato O (12)
|
NTLAC |
55.56 |
57.00 |
1.44 |
| 3 |
Jaya, Made A (12)
|
NTLAC |
56.98 |
57.19 |
0.21 |
| 4 |
Xiao, Leonardo Y (12)
|
NTLAC |
58.03 |
57.59 |
-0.44 |
| 5 |
Volpe, Christopher J (12)
|
NTFAST |
59.64 |
58.61 |
-1.03 |
| 6 |
Xu, Sherwin M (12)
|
NTLAC |
59.23 |
1:00.35 |
1.12 |
| 7 |
Zheng, Viktor F (12)
|
NTLAC |
1:01.05 |
1:00.57 |
-0.48 |
| 8 |
Zhang, William T (11)
|
NTNTN |
1:02.37 |
1:00.90 |
-1.47 |
| 9 |
Fang, Yifan (12)
|
NTLAC |
1:01.30 |
1:01.11 |
-0.19 |
| 10 |
Kolody, Brendan (11)
|
NTLAC |
1:01.27 |
1:01.16 |
-0.11 |
| 11 |
Alvarez Arteaga, Derek (12)
|
NTUN |
1:05.20 |
1:01.78 |
-3.42 |
| 12 |
Owens, Connor P (12)
|
NTMTRO |
1:05.44 |
1:02.14 |
-3.30 |
| 13 |
Roybal, Hudson L (11)
|
NTLAC |
1:02.25 |
1:02.26 |
0.01 |
| 14 |
Cormack, Eason C (12)
|
NTNTN |
1:01.80 |
1:02.41 |
0.61 |
| 15 |
Jones, Dallas J (12)
|
NTLAC |
1:02.56 |
1:02.42 |
-0.14 |
| 16 |
Cortinas, Sebastian N (12)
|
NTLAC |
1:05.55 |
1:02.75 |
-2.80 |
| 17 |
Wester, Christian M (11)
|
NTLAC |
1:14.03 |
1:02.76 |
-11.27 |
| 18 |
Palmer, Daniel J (11)
|
NTLAC |
1:04.78 |
1:03.53 |
-1.25 |
| 19 |
Batuna, Jason C (12)
|
NTLAC |
1:03.27 |
1:04.11 |
0.84 |
| 20 |
Heldenbrand, Dylan F (11)
|
NTNTN |
1:03.69 |
1:04.25 |
0.56 |
| 21 |
Murthy, Dhanush (11)
|
NTLAC |
1:08.36 |
1:05.60 |
-2.76 |
| 22 |
Jackson, Grant J (12)
|
NTLAC |
1:14.09 |
1:05.86 |
-8.23 |
| 23 |
Wright, Simon R (12)
|
NTNTN |
1:06.33 |
1:07.15 |
0.82 |
| 24 |
Chiang, Tyler B (11)
|
NTLAC |
1:06.14 |
1:08.08 |
1.94 |
| 25 |
Currey, William P (12)
|
NTLAC |
1:05.48 |
1:08.18 |
2.70 |
| 26 |
Poe, Ian (12)
|
NTLAC |
1:09.67 |
1:12.38 |
2.71 |