| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moore, Caitlyn N (8)
|
VANOVA |
41.48 |
40.21 |
-1.27 |
| 2 |
Bradley, Macie J (8)
|
VAUN-N |
44.04 |
41.40 |
-2.64 |
| 3 |
Wood, Raegan M (8)
|
VARAYS |
42.83 |
41.56 |
-1.27 |
| 4 |
Tingen, Bailey S (8)
|
VAWAC |
41.92 |
43.08 |
1.16 |
| 5 |
Muhlenkamp, Allyson J (8)
|
VARAYS |
55.00 |
45.59 |
-9.41 |
| 6 |
Costello, Kaylee J (8)
|
VADC |
49.28 |
46.97 |
-2.31 |
| 7 |
Williamson, Annika R (8)
|
VASRVA |
50.74 |
47.93 |
-2.81 |
| 8 |
Robertson, Caroline C (7)
|
VAWAC |
47.19 |
49.13 |
1.94 |
| 9 |
Bontrager, Peyton R (8)
|
VASRVA |
52.36 |
54.05 |
1.69 |
| 10 |
May, Finley K (8)
|
VAQSTS |
53.73 |
54.54 |
0.81 |
| 11 |
McFarlane, Lauren E (8)
|
UN-V |
51.52 |
54.70 |
3.18 |
| 12 |
Currier, Hailey M (6)
|
VAWAC |
1:01.23 |
56.77 |
-4.46 |
| 13 |
Mullinax, Rosina G (8)
|
VADC |
|
58.79 |
- |
| 14 |
Cannedy, Khloe L (6)
|
VARAYS |
1:05.33 |
59.37 |
-5.96 |
| 15 |
Berling, Hannah G (7)
|
VADC |
|
1:03.15 |
- |
| 16 |
Smith, Madelynn E (7)
|
VASRVA |
1:05.93 |
1:03.19 |
-2.74 |
| 17 |
Goad, Macie A (7)
|
VAQSTS |
1:02.91 |
1:06.13 |
3.22 |
| 18 |
Scullin, Sienna A (8)
|
VARAYS |
32.00 |
1:08.79 |
36.79 |
|
Yoon, Bandy (7)
|
VADC |
|
DQ |
- |
|
Dodd, Margot B (8)
|
VASRVA |
48.04 |
DQ |
- |