| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hall, Jay-Colin S (12)
|
GULAP |
28.67 |
29.45 |
0.78 |
| 2 |
Barrera, Juan S (12)
|
GUHCAP |
34.99 |
34.25 |
-0.74 |
| 3 |
Soto, Eduardo A (11)
|
GUCOOG |
35.86 |
34.59 |
-1.27 |
| 4 |
Grolemund, Liam J (12)
|
GUSSS |
38.12 |
35.39 |
-2.73 |
| 5 |
Paelicke, Ian H (12)
|
GUSSS |
37.44 |
37.48 |
0.04 |
| 6 |
Melo, Edgar A (11)
|
GUHCAP |
37.98 |
38.87 |
0.89 |
| 7 |
Nielsen, Hayden T (11)
|
GUSHRK |
38.60 |
38.96 |
0.36 |
| 8 |
Lopez Turley, Alejandro D (12)
|
GUCOOG |
39.77 |
40.00 |
0.23 |
| 9 |
Stratton, Mac D (11)
|
GUCOOG |
39.72 |
40.41 |
0.69 |
| 10 |
Grolemund, Samuel W (11)
|
GUSSS |
47.04 |
44.26 |
-2.78 |
| 11 |
Givens, Noah W (11)
|
GUSHRK |
46.09 |
45.19 |
-0.90 |
| 12 |
De Los Angeles, Sebastian (11)
|
GUCOOG |
57.48 |
49.74 |
-7.74 |
| 13 |
Mounts, Christian M (11)
|
GUTCAP |
1:00.21 |
51.15 |
-9.06 |
| 14 |
Ganapathy, Auyush (11)
|
GUSHRK |
1:05.86 |
1:03.44 |
-2.42 |
|
Chen, David (11)
|
GUSSS |
|
DQ |
- |
|
Anderson, Kai A (11)
|
GUSSS |
1:06.31 |
NS |
- |