| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Koroma, Sophie I (11)
|
PVNCAP |
29.24 |
28.52 |
-0.72 |
| 2 |
DeGuenther, Emory B (11)
|
VANOVA |
29.62 |
29.13 |
-0.49 |
| 3 |
Mulkey, Anne M (11)
|
PVNCAP |
31.30 |
29.85 |
-1.45 |
| 4 |
Majeed-Hall, Aliyah M (11)
|
PVNCAP |
30.57 |
30.19 |
-0.38 |
| 5 |
Anderson, Samantha R (11)
|
MRLIAC |
31.46 |
30.35 |
-1.11 |
| 6 |
Armstrong, Kiersten R (11)
|
VANOVA |
31.11 |
30.91 |
-0.20 |
| 7 |
Wu, Natalie (11)
|
MRLIAC |
31.74 |
30.95 |
-0.79 |
| 8 |
Lacy, Katherine S (11)
|
VANOVA |
31.03 |
30.96 |
-0.07 |
| 9 |
Heath, Ariana L (11)
|
PVNCAP |
31.25 |
31.00 |
-0.25 |
| 10 |
Barnes, Addison A (11)
|
VANOVA |
34.18 |
34.11 |
-0.07 |