| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carvalho, Gabriela (11)
|
VANOVA |
28.04 |
27.14 |
-0.90 |
| 2 |
Callihan, Catherine B (11)
|
VANOVA |
29.09 |
27.20 |
-1.89 |
| 3 |
Koroma, Sophie I (11)
|
PVNCAP |
26.44 |
27.27 |
0.83 |
| 4 |
Heath, Briana L (11)
|
PVNCAP |
27.97 |
27.40 |
-0.57 |
| 5 |
Heath, Ariana L (11)
|
PVNCAP |
28.63 |
28.29 |
-0.34 |
| 6 |
Tittermary, Katherine J (11)
|
VANOVA |
29.57 |
28.40 |
-1.17 |
| 7 |
So, Leni A (11)
|
MRLIAC |
28.74 |
28.41 |
-0.33 |
| 8 |
Connor, Kaitlin G (11)
|
MRLIAC |
28.97 |
28.60 |
-0.37 |
| 9 |
Maccaroni, Vivi M (11)
|
VANOVA |
29.35 |
28.94 |
-0.41 |
| 10 |
Mulkey, Anne M (11)
|
PVNCAP |
29.23 |
29.15 |
-0.08 |
| 11 |
McDonald, Cara B (11)
|
MRLIAC |
31.09 |
30.17 |
-0.92 |
| 12 |
Doran, Molly K (11)
|
MRLIAC |
32.01 |
30.82 |
-1.19 |