| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bechtel, Lyla R (10)
|
MACHEL |
52.67 |
44.16 |
-8.51 |
| 2 |
D'Alesio, Jenna (10)
|
MACAA |
54.74 |
45.30 |
-9.44 |
| 3 |
Ridge, Taryn L (10)
|
MAWAC |
53.15 |
47.10 |
-6.05 |
| 4 |
Rogers, Emma L (10)
|
MAKIDS |
54.01 |
47.57 |
-6.44 |
| 5 |
Moschella, Alexandra J (10)
|
MAWA |
50.50 |
47.75 |
-2.75 |
| 6 |
Hogas, Sophia E (10)
|
MASTRM |
50.85 |
47.81 |
-3.04 |
| 7 |
Taddeo, Giavanna C (10)
|
MALIFE |
49.81 |
50.95 |
1.14 |
| 8 |
Cestare, Miranda J (10)
|
MAJW |
56.74 |
51.49 |
-5.25 |
| 9 |
Kiss, Katelyn (10)
|
MAKIDS |
51.92 |
52.20 |
0.28 |
| 10 |
Sheldon, Samantha M (10)
|
MAJRAY |
54.56 |
52.68 |
-1.88 |
| 11 |
McCauley, Catherine J (10)
|
MATWAC |
55.98 |
53.04 |
-2.94 |
| 12 |
Marchenko, Alina (10)
|
MALIFE |
52.47 |
55.57 |
3.10 |
| 13 |
Whitten, Georgia (10)
|
MALIFE |
1:05.13 |
57.47 |
-7.66 |
| 14 |
May, Piper C (10)
|
MAMSA |
1:02.81 |
57.83 |
-4.98 |
| 15 |
Findley, Caribeean P (10)
|
MAJRAY |
55.52 |
57.94 |
2.42 |
| 16 |
Gaj, Catherine A (10)
|
MAJW |
55.94 |
58.04 |
2.10 |
|
Espinoza, Alena M (10)
|
MAGPAC |
1:10.71 |
DQ |
- |
|
Dabrowski, Sarah R (10)
|
MAKA |
51.66 |
NS |
- |
|
Reddy, Aashika (10)
|
MAJW |
57.32 |
NS |
- |
|
Gonnella, Lila G (10)
|
MAGAAC |
50.62 |
NS |
- |
|
Noval, Paige E (10)
|
MABRY |
56.04 |
NS |
- |