| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lepley, Brooks P (10)
|
MDLBA |
47.53 |
43.86 |
-3.67 |
| 2 |
Plaisted, Harrison T (10)
|
MDBAAC |
46.82 |
44.04 |
-2.78 |
| 3 |
Deremeik, Tucker J (9)
|
MDLBA |
44.56 |
44.48 |
-0.08 |
| 4 |
Francis, Chase P (10)
|
MDLBA |
46.80 |
44.67 |
-2.13 |
| 5 |
Reed, Richard W (10)
|
MDNBAC |
45.09 |
44.91 |
-0.18 |
| 6 |
Sayre, Patrick R (10)
|
MDFSC |
47.35 |
45.16 |
-2.19 |
| 7 |
Bowers, Tyler L (9)
|
MDFSC |
45.25 |
45.66 |
0.41 |
| 8 |
Hazlehurst, Aidan (10)
|
MDNBAC |
46.43 |
45.76 |
-0.67 |
| 9 |
Cason, Musaa B (10)
|
MDBCSC |
45.98 |
45.77 |
-0.21 |
| 10 |
Wong, Kieran j (10)
|
MDACA |
45.89 |
45.82 |
-0.07 |
| 11 |
Shi, Justin W (9)
|
MDRAC |
46.41 |
45.88 |
-0.53 |
| 12 |
Studnicki, Matthew R (10)
|
MDBAAC |
48.16 |
46.16 |
-2.00 |
| 13 |
Dieveney, Charles T (9)
|
MDLBA |
47.72 |
46.64 |
-1.08 |
| 14 |
Reed, Bennett C (10)
|
MDGBSA |
47.89 |
46.65 |
-1.24 |
| 15 |
Moran, Connor W (10)
|
MDFSC |
47.05 |
47.34 |
0.29 |
| 16 |
Olszyk, Gabriel A (10)
|
MDBAAC |
46.99 |
47.41 |
0.42 |
| 17 |
Lisiewski, Adrian Z (9)
|
MDBAAC |
46.13 |
47.49 |
1.36 |
| 18 |
Fisher, Aidan T (10)
|
MDACA |
48.01 |
47.57 |
-0.44 |
| 19 |
Kim, Victor S (9)
|
MDACA |
47.94 |
48.14 |
0.20 |
| 20 |
Wiseman, Maxwell C (10)
|
MDLBA |
47.86 |
48.28 |
0.42 |
| 21 |
Cabbagestalk Jr., Travone J (10)
|
MDBCSC |
48.03 |
48.33 |
0.30 |
| 22 |
Farvolden, Sullivan G (9)
|
MDNBAC |
48.87 |
50.95 |
2.08 |
|
Roberts, Justin C (9)
|
MDNBAC |
48.04 |
DQ |
- |
|
Mason, Jaylin T (9)
|
MDFMSC |
47.05 |
DQ |
- |