| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sellars, Elijah A (11)
|
NCQCD |
34.67 |
34.65 |
-0.02 |
| 2 |
Hardy, Gabrian G (11)
|
NCMAC |
38.52 |
35.29 |
-3.23 |
| 3 |
Sidden, Wyatt R (11)
|
NCEAC |
38.47 |
38.31 |
-0.16 |
| 4 |
Hohm, Owen J (11)
|
NCATOM |
38.75 |
38.52 |
-0.23 |
| 5 |
Schwarzkopf, Connor A (11)
|
NCMAC |
41.66 |
38.78 |
-2.88 |
| 6 |
Harris, Joshua B (11)
|
NCWOW |
39.61 |
39.29 |
-0.32 |
| 7 |
Dugan, Tyler N (11)
|
NCMAC |
42.69 |
40.52 |
-2.17 |
| 8 |
Greathouse, Thomas A (11)
|
NCMAC |
44.42 |
40.74 |
-3.68 |
| 9 |
Hart III, Neil J (11)
|
NCMAC |
42.41 |
41.26 |
-1.15 |
| 10 |
Vogt, Jonathan M (11)
|
NCEAC |
41.87 |
43.93 |
2.06 |
| 11 |
Papaioannou, Stefanos (11)
|
NCEAC |
44.82 |
44.17 |
-0.65 |