| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hastings, Lucy (8)
|
NCWOW |
25.92 |
24.46 |
-1.46 |
| 2 |
Nicholson, Madelyn G (8)
|
NCWOW |
25.40 |
25.16 |
-0.24 |
| 3 |
Herring, Summer S (7)
|
NCBAC |
31.57 |
28.14 |
-3.43 |
| 4 |
Alexander, Jalyn L (7)
|
NCGFSC |
30.70 |
28.24 |
-2.46 |
| 5 |
Cumbee, Phoebe A (8)
|
NCBAC |
32.79 |
28.36 |
-4.43 |
| 6 |
House, Asheford R (8)
|
NCUN |
28.87 |
29.06 |
0.19 |
| 7 |
Carroll, Charlotte A (8)
|
NCWOW |
30.05 |
29.28 |
-0.77 |
| 8 |
Langway, Lillian C (8)
|
SCCAC |
29.63 |
29.33 |
-0.30 |
| 9 |
Ivins, Grace M (8)
|
NCUN |
31.67 |
29.45 |
-2.22 |
| 10 |
Blair, Abigail J (7)
|
NCCFAC |
|
30.01 |
- |
| 11 |
Spencer, Summer R (8)
|
NCCFAC |
29.54 |
30.05 |
0.51 |
| 12 |
Charkoudian, Sarah N (7)
|
NCCFAC |
35.06 |
31.58 |
-3.48 |
| 13 |
Witmer, Gentry L (6)
|
NCUN |
29.88 |
31.67 |
1.79 |
|
Smithwick, Harper E (7)
|
NCGFSC |
|
DQ |
- |
|
Hohl, Ava M (8)
|
NCCFAC |
|
DQ |
- |
|
Nester, Addison S (7)
|
NCBAC |
38.83 |
DQ |
- |
|
McMullan, Annabelle B (4)
|
NCBAC |
|
DQ |
- |
|
Garver, Meghan E (8)
|
NCBAC |
|
DQ |
- |
|
Senter, Ainsley J (8)
|
NCCFAC |
27.10 |
NS |
- |