| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Neu, Nathan A (10)
|
NCCFAC |
30.05 |
29.19 |
-0.86 |
| 2 |
Clayton, Thomas J (10)
|
NCGFSC |
29.28 |
29.27 |
-0.01 |
| 3 |
Delos Santos, Joshua B (10)
|
NCGFSC |
29.32 |
29.41 |
0.09 |
| 4 |
Payne, Charlie W (10)
|
NCGFSC |
30.32 |
29.52 |
-0.80 |
| 5 |
Butner, Stephen A (10)
|
NCWOW |
32.13 |
31.72 |
-0.41 |
| 6 |
Walcott, Noah S (10)
|
NCGFSC |
33.08 |
33.51 |
0.43 |
| 7 |
Harris, Joshua B (10)
|
NCWOW |
34.03 |
33.65 |
-0.38 |
| 8 |
Petroff, Behrett B (9)
|
NCWOW |
35.34 |
34.40 |
-0.94 |
| 9 |
Widner, Preston R (9)
|
SCCAC |
37.83 |
34.77 |
-3.06 |
| 10 |
Mendieta, Logan L (9)
|
SCCAC |
35.95 |
35.47 |
-0.48 |
| 11 |
Mayo, Horace H (8)
|
NCWOW |
37.71 |
35.66 |
-2.05 |
| 12 |
Huggins, Linus A (10)
|
NCCFAC |
36.57 |
35.67 |
-0.90 |
| 13 |
Boehmer, Jakob A (9)
|
NCBAC |
37.90 |
35.95 |
-1.95 |
| 14 |
Spencer, Slater W (7)
|
NCCFAC |
37.55 |
36.13 |
-1.42 |
| 15 |
Jessup, Cooper J (10)
|
NCBAC |
39.83 |
37.16 |
-2.67 |
| 16 |
Kraus, William A (10)
|
NCWOW |
43.06 |
37.96 |
-5.10 |
| 17 |
Boehmer, Oliver R (9)
|
NCBAC |
36.18 |
38.13 |
1.95 |
| 18 |
Keena, Stephen M (10)
|
NCBAC |
38.96 |
38.53 |
-0.43 |
| 19 |
Hudson, Charlie N (9)
|
NCWOW |
38.96 |
38.65 |
-0.31 |
| 20 |
Pascov, George V (9)
|
SCCAC |
39.72 |
39.01 |
-0.71 |
| 21 |
Pike, Dustin L (9)
|
NCUNAF |
|
39.52 |
- |
| 22 |
Herring, Sawyer D (10)
|
NCBAC |
44.96 |
40.57 |
-4.39 |
| 23 |
Eichenberger, Bryce A (10)
|
SCMBR |
44.61 |
40.68 |
-3.93 |
| 24 |
Blair, Bryan J (9)
|
NCCFAC |
39.12 |
40.99 |
1.87 |
| 25 |
McWhorter, Maddox F (10)
|
SCMBR |
42.49 |
42.44 |
-0.05 |
| 26 |
Taylor, Clayton H (8)
|
NCBAC |
43.15 |
42.55 |
-0.60 |
| 27 |
Zaki, Youssef R (10)
|
SCMBR |
|
43.52 |
- |
| 27 |
Derry, Gavin M (7)
|
NCWOW |
42.56 |
43.52 |
0.96 |
| 29 |
Savage, Thomas G (9)
|
NCWOW |
56.47 |
45.87 |
-10.60 |
| 30 |
Walcott, Daniel J (7)
|
NCGFSC |
49.57 |
47.61 |
-1.96 |
| 31 |
Ingram, Norman P (10)
|
NCBAC |
|
51.16 |
- |
| 32 |
Lac, Takoda Y (6)
|
NCWOW |
52.08 |
53.68 |
1.60 |
| 33 |
Dou, Yihao (7)
|
SCCAC |
57.50 |
53.92 |
-3.58 |
| 34 |
Reaves, Chace T (7)
|
NCWOW |
52.24 |
59.60 |
7.36 |
| 35 |
Davis, William B (6)
|
NCBAC |
1:04.65 |
1:01.40 |
-3.25 |
| 36 |
Ingram, Andrew A (7)
|
NCBAC |
|
1:06.22 |
- |
| 37 |
Morris Jr., Charles A (6)
|
NCBAC |
|
1:08.28 |
- |
| 38 |
Lanier, Isaac S (6)
|
NCCFAC |
|
1:10.10 |
- |
| 39 |
Forbes, William G (7)
|
NCUN |
1:05.44 |
1:16.35 |
10.91 |
|
Tompkins, Jonathan H (10)
|
NCWOW |
42.06 |
NS |
- |
|
Lanier, Luke B (7)
|
NCCFAC |
|
NS |
- |
|
Dettman, Jonah H (10)
|
NCGFSC |
37.50 |
NS |
- |