| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Riley, Seth A (12)
|
SCMBR |
33.43 |
31.60 |
-1.83 |
| 2 |
Clayton, William F (12)
|
NCGFSC |
37.05 |
36.63 |
-0.42 |
| 3 |
Torsone, Bryce G (11)
|
NCGFSC |
37.85 |
37.19 |
-0.66 |
| 4 |
Kinmartin, Mason C (12)
|
SCMBR |
37.81 |
37.31 |
-0.50 |
| 5 |
Walcott, Benjamin J (12)
|
NCGFSC |
41.31 |
38.47 |
-2.84 |
| 6 |
Ive, Jethro T (12)
|
NCWOW |
40.61 |
39.05 |
-1.56 |
| 7 |
Brown, Lawson W (11)
|
NCCFAC |
40.40 |
39.70 |
-0.70 |
| 7 |
Arredondo, Nathan B (11)
|
NCWOW |
39.69 |
39.70 |
0.01 |
| 9 |
Reiniche, Ryan J (11)
|
NCWOW |
40.35 |
40.40 |
0.05 |
| 10 |
House, William E (11)
|
NCUN |
40.11 |
40.76 |
0.65 |
| 11 |
Boudreau, Ryan M (11)
|
SCCAC |
41.04 |
40.91 |
-0.13 |
| 12 |
Westerbeek, Jase H (12)
|
NCUNAF |
|
40.95 |
- |
| 13 |
Derwin, Nathaniel T (12)
|
NCGFSC |
44.93 |
41.34 |
-3.59 |
| 14 |
Desnoyers, Tyce A (12)
|
SCMBR |
42.87 |
41.83 |
-1.04 |
| 15 |
DiGuiseppe, Julian M (12)
|
NCBAC |
41.99 |
42.25 |
0.26 |
| 16 |
Brown, Noah D (12)
|
NCCFAC |
43.49 |
42.67 |
-0.82 |
| 17 |
Artz, Samuel M (11)
|
SCCAC |
40.70 |
44.09 |
3.39 |
| 18 |
Mejouev, Maxim (12)
|
NCWOW |
44.91 |
44.22 |
-0.69 |
| 19 |
McDonald, Ray D (11)
|
NCCFAC |
48.75 |
44.85 |
-3.90 |
| 20 |
Ausband, Laith J (11)
|
NCUN |
43.22 |
45.48 |
2.26 |
| 21 |
Miller, Greyson F (12)
|
NCBAC |
48.01 |
45.85 |
-2.16 |
| 22 |
Tillery, Wade H (12)
|
NCWOW |
44.95 |
46.26 |
1.31 |
| 23 |
Eder, Ryker P (11)
|
NCUN |
45.50 |
46.81 |
1.31 |
| 24 |
Park, Dylan T (11)
|
NCWOW |
49.67 |
49.23 |
-0.44 |
| 25 |
Hardison, Eli C (12)
|
NCUNAF |
59.71 |
51.07 |
-8.64 |
| 26 |
Bullard, Benjamin M (11)
|
NCUN |
|
51.09 |
- |
| 27 |
Gedman, Isaac N (11)
|
NCBAC |
58.09 |
56.66 |
-1.43 |
| 28 |
Henrick, Ryan C (11)
|
SCMBR |
|
58.02 |
- |
| 29 |
McGhee, Jackson T (11)
|
NCBAC |
1:13.45 |
1:08.20 |
-5.25 |
|
Upadhyay, Apurva N (11)
|
NCGFSC |
49.83 |
NS |
- |
|
Pendleton, Zackary A (11)
|
NCGFSC |
48.34 |
NS |
- |