| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Joy, Madison A (12)
|
NCWOW |
2:26.94 |
2:26.39 |
-0.55 |
| 2 |
Forbes, Abigail R (12)
|
NCUN |
2:35.48 |
2:35.11 |
-0.37 |
| 3 |
Weigand, Hailey J (12)
|
NCWOW |
2:39.50 |
2:37.25 |
-2.25 |
| 4 |
Britton, Jaceil A (12)
|
NCCFAC |
2:49.83 |
2:39.29 |
-10.54 |
| 5 |
Boudreau, Arika L (12)
|
SCMBR |
|
2:42.50 |
- |
| 6 |
Ludeman, Alison g (12)
|
SCMBR |
3:12.93 |
2:43.55 |
-29.38 |
| 7 |
Witmer, Keener L (12)
|
NCUN |
2:42.21 |
2:43.86 |
1.65 |
| 8 |
Jacquemin, Madison G (11)
|
SCCAC |
2:54.33 |
2:45.90 |
-8.43 |
| 9 |
Steele, Eliana C (12)
|
NCWOW |
2:48.76 |
2:46.38 |
-2.38 |
| 10 |
Williams, Lexi M (11)
|
NCWOW |
2:44.92 |
2:48.44 |
3.52 |
| 11 |
Metzinger, Mya Z (11)
|
NCWOW |
2:46.77 |
2:50.10 |
3.33 |
| 12 |
Pierson, Sarah S (12)
|
NCWOW |
2:48.13 |
2:51.56 |
3.43 |
| 13 |
Williams, Aley E (12)
|
NCBAC |
2:52.88 |
2:52.19 |
-0.69 |
| 14 |
Mitchell, Lily K (12)
|
NCGFSC |
2:50.56 |
2:52.77 |
2.21 |
| 15 |
Brown, Olivia C (12)
|
NCCFAC |
2:56.97 |
2:52.86 |
-4.11 |
| 16 |
Robideaux, Chandra R (11)
|
SCCAC |
|
2:53.26 |
- |
| 17 |
Davis, Mary R (12)
|
NCBAC |
|
2:53.55 |
- |
| 18 |
Jansky, Savannah L (11)
|
SCCAC |
2:51.87 |
2:56.20 |
4.33 |
| 19 |
Backman, Emmie N (12)
|
NCCFAC |
|
2:56.60 |
- |
| 20 |
McNamara, Cienna R (12)
|
NCWOW |
2:55.20 |
2:57.75 |
2.55 |
| 21 |
Reaves, Camren G (11)
|
NCWOW |
2:58.31 |
2:58.25 |
-0.06 |
| 22 |
Harvey, Sara P (12)
|
NCCFAC |
|
3:02.25 |
- |
| 23 |
Washko, Mckenzie G (11)
|
SCMBR |
3:08.60 |
3:04.93 |
-3.67 |
| 24 |
Hall, Sophia M (12)
|
NCCFAC |
|
3:06.12 |
- |
| 25 |
Burroughs, Juliet J (11)
|
NCCFAC |
|
3:11.35 |
- |
| 26 |
Santucci, Skylar (11)
|
NCWOW |
|
3:21.81 |
- |
| 27 |
Farrell, Kennedy E (11)
|
NCCFAC |
|
3:30.90 |
- |
| 28 |
Sterner, Adalyn G (11)
|
NCWOW |
3:36.40 |
3:44.29 |
7.89 |
|
Senter, Ella N (11)
|
NCCFAC |
|
DQ |
- |
|
Holland, Caroline R (12)
|
NCUNAF |
|
NS |
- |