| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Anastasia L (17)
|
NCRSA |
56.24 |
57.82 |
1.58 |
| 2 |
Clack, Carrington L (17)
|
NCRSA |
55.22 |
58.20 |
2.98 |
| 3 |
Gooch, Ellen J (18)
|
NCHAC |
56.36 |
58.47 |
2.11 |
| 4 |
McGrath, Natalie R (17)
|
NCMOR |
55.87 |
59.32 |
3.45 |
| 5 |
Van Luvender, Harley E (17)
|
NCRSA |
56.44 |
59.47 |
3.03 |
| 6 |
Blair, Kathryn E (17)
|
NCCAT |
57.86 |
1:00.27 |
2.41 |
| 7 |
Wisz, Marie E (17)
|
NCMOR |
59.24 |
1:00.94 |
1.70 |
| 8 |
Kempf, Stella M (17)
|
NCRSA |
55.05 |
1:01.16 |
6.11 |
| 9 |
Carew, Emma K (17)
|
NCHAC |
59.51 |
1:02.82 |
3.31 |
| 10 |
Leischner, Peyton T (17)
|
NCMOR |
59.53 |
1:05.47 |
5.94 |
| 11 |
Montefiori, Natalie K (17)
|
NCCAT |
1:03.37 |
1:07.52 |
4.15 |
| 12 |
Boyd, Jenny K (17)
|
NCVACB |
1:11.92 |
1:09.57 |
-2.35 |
|
Hasmann, Morgan D (17)
|
NCCAT |
1:06.56 |
NS |
- |
|
Link, Madison G (17)
|
NCRSA |
56.90 |
NS |
- |
|
Denton, Elizabeth A (18)
|
NCVACB |
1:00.54 |
NS |
- |