| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Gunner L (11)
|
NCCVAC |
36.31 |
34.47 |
-1.84 |
| 2 |
Hedrick, Noah A (11)
|
NCCVAC |
37.03 |
35.96 |
-1.07 |
| 3 |
Johnson, Carter D (12)
|
NCGG |
41.37 |
40.32 |
-1.05 |
| 4 |
Bartlett, Jack W (12)
|
NCCVAC |
41.80 |
40.71 |
-1.09 |
| 5 |
Robinson, Jonah R (11)
|
NCGG |
43.84 |
40.76 |
-3.08 |
| 6 |
Olguin-Abreu, David F (11)
|
NCCVAC |
44.09 |
44.82 |
0.73 |
| 7 |
Williams, Jaasiel L (12)
|
NCGG |
46.61 |
45.52 |
-1.09 |
| 8 |
Kesterson, Christopher T (11)
|
NCRCST |
46.79 |
45.77 |
-1.02 |
| 9 |
Ramirez, Daniel A (12)
|
NCCVAC |
|
55.29 |
- |
| 10 |
Brantley, Landon N (11)
|
NCWA |
1:02.93 |
59.60 |
-3.33 |
| 11 |
Morales, Joshua A (11)
|
NCGG |
57.92 |
1:00.20 |
2.28 |
|
Cable, Euan A (11)
|
NCGG |
49.01 |
DQ |
- |
|
Torres, Jetiel J (12)
|
NCAQTC |
42.34 |
NS |
- |
|
Moseley, Josiah L (11)
|
NCCVAC |
1:02.50 |
NS |
- |