| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Niebauer, Drew E (12)
|
SEBSC |
36.02 |
32.55 |
-3.47 |
| 2 |
Czar, Matthew E (12)
|
NCHAC |
32.86 |
32.67 |
-0.19 |
| 3 |
Burton, Bence W (12)
|
NCEAC |
33.61 |
33.31 |
-0.30 |
| 4 |
Reiniche, Ryan J (12)
|
NCWOW |
36.37 |
35.57 |
-0.80 |
| 5 |
Lipscomb, Michael E (11)
|
SEBSC |
36.24 |
36.38 |
0.14 |
| 6 |
Burton, Tyler A (11)
|
NCEAC |
36.91 |
36.87 |
-0.04 |
| 7 |
Ryan, Braden (12)
|
NCTEAM |
38.11 |
37.02 |
-1.09 |
| 8 |
Morgan, Noah S (11)
|
NCSFSC |
39.29 |
38.47 |
-0.82 |
| 9 |
Hoskins IV, Robert E (11)
|
NCEAC |
|
40.42 |
- |
| 10 |
Popescu, Jonathan D (12)
|
NCHAC |
40.42 |
41.91 |
1.49 |
| 11 |
Carnahan, Declan A (11)
|
NCHAC |
46.99 |
44.09 |
-2.90 |
| 12 |
Croxton, Dylan J (11)
|
VAUN-3 |
47.72 |
44.18 |
-3.54 |
| 13 |
Neighbors, Arthur J (11)
|
NCSFSC |
47.51 |
49.37 |
1.86 |
| 14 |
Tranel, Taylon K (11)
|
NCNEAL |
55.48 |
53.63 |
-1.85 |
| 15 |
Tompkins, Jonathan H (11)
|
NCWOW |
1:03.20 |
1:05.21 |
2.01 |
|
Conklin, Brandt C (12)
|
NCSFSC |
38.95 |
NS |
- |
|
Schmitz, Samuel M (11)
|
NCNEAL |
54.00 |
NS |
- |
|
Sequeiros, Sebastian (11)
|
MRNYSA |
52.22 |
NS |
- |
|
Liu, Jared (11)
|
MRNYSA |
38.09 |
NS |
- |