| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Niebauer, Drew E (12)
|
SEBSC |
31.47 |
29.85 |
-1.62 |
| 2 |
Conklin, Brandt C (12)
|
NCSFSC |
31.10 |
30.37 |
-0.73 |
| 3 |
Austin, Thaddeus E (12)
|
NCEAC |
30.86 |
30.86 |
0.00 |
| 4 |
Czar, Matthew E (12)
|
NCHAC |
31.36 |
31.07 |
-0.29 |
| 5 |
Reiniche, Ryan J (12)
|
NCWOW |
33.12 |
32.96 |
-0.16 |
| 6 |
Morgan, Noah S (11)
|
NCSFSC |
35.81 |
33.56 |
-2.25 |
| 7 |
Hoskins IV, Robert E (11)
|
NCEAC |
|
35.08 |
- |
| 8 |
Croxton, Dylan J (11)
|
VAUN-3 |
37.94 |
36.67 |
-1.27 |
| 9 |
Carnahan, Declan A (11)
|
NCHAC |
36.06 |
37.09 |
1.03 |
| 10 |
Popescu, Jonathan D (12)
|
NCHAC |
38.87 |
38.89 |
0.02 |
| 11 |
Eisele, Andrew M (11)
|
VAUN-3 |
40.23 |
39.55 |
-0.68 |
| 12 |
Tranel, Taylon K (11)
|
NCNEAL |
43.00 |
41.38 |
-1.62 |
| 13 |
Neighbors, Arthur J (11)
|
NCSFSC |
39.11 |
42.39 |
3.28 |
| 14 |
Tompkins, Jonathan H (11)
|
NCWOW |
42.65 |
46.21 |
3.56 |
|
Mochalov, Daniil L (11)
|
VADYST |
|
NS |
- |
|
Vogt, Jonathan M (11)
|
NCEAC |
38.92 |
NS |
- |