| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zapler, Ezra N (11)
|
NCECA |
32.33 |
31.62 |
-0.71 |
| 2 |
Villalobos, Adrian J (12)
|
NCRSA |
32.92 |
32.67 |
-0.25 |
| 3 |
Schaper, Calvin O (11)
|
NCCAT |
42.06 |
37.40 |
-4.66 |
| 4 |
Leisten, Elijah Q (12)
|
NCRSA |
42.59 |
39.88 |
-2.71 |
| 5 |
DeSimone, Benjamin R (11)
|
NCCAT |
44.76 |
42.69 |
-2.07 |
| 6 |
Kim, Joshua R (11)
|
NCCAT |
47.94 |
43.03 |
-4.91 |
| 7 |
Bullard, Andrew W (11)
|
NCECA |
45.21 |
44.24 |
-0.97 |
| 8 |
Hignite, Ian G (11)
|
NCRSA |
48.33 |
44.51 |
-3.82 |
| 9 |
Johnson, Samuel T (12)
|
NCECA |
52.99 |
45.65 |
-7.34 |
| 10 |
Bondo, Christopher L (12)
|
NCRSA |
47.42 |
47.81 |
0.39 |
| 11 |
Michalowicz, Jason B (11)
|
NCECA |
52.69 |
49.35 |
-3.34 |
| 12 |
Wuthrich, Colin E (11)
|
NCHAC |
53.72 |
50.80 |
-2.92 |
| 13 |
Walker, Maxwell T (12)
|
NCHAC |
51.26 |
52.62 |
1.36 |
|
Ray, Charles W (11)
|
NCCAT |
1:10.16 |
NS |
- |