| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Collins, Daisy B (10)
|
NCNCAC |
40.69 |
39.71 |
-0.98 |
| 2 |
Adekoya, Oyinkansola O (10)
|
NCGCY |
43.07 |
40.70 |
-2.37 |
| 3 |
Palmer, Jaylin A (10)
|
NCGCY |
43.85 |
41.61 |
-2.24 |
| 4 |
Liu, Reina R (9)
|
NCGCY |
43.06 |
42.09 |
-0.97 |
| 5 |
Wang, Sophie W (9)
|
NCGCY |
51.83 |
43.06 |
-8.77 |
| 6 |
Brock, Yari E (9)
|
NCNCAC |
44.66 |
43.09 |
-1.57 |
| 7 |
Buttar, Amira A (10)
|
NCLOY |
45.98 |
43.70 |
-2.28 |
| 8 |
Biswas, Emma A (10)
|
NCNCAC |
45.88 |
44.01 |
-1.87 |
| 9 |
Testa, Madeline C (10)
|
NCSGSA |
49.96 |
44.60 |
-5.36 |
| 10 |
Henderson, Catelyn G (10)
|
NCGCY |
45.52 |
45.23 |
-0.29 |
| 11 |
Ma, Runhui (10)
|
NCNCAC |
1:00.99 |
46.14 |
-14.85 |
| 12 |
Kallepalli, Anya (10)
|
NCNCAC |
47.68 |
47.85 |
0.17 |
| 13 |
Hicks, Jozi E (10)
|
NCUN |
53.00 |
47.86 |
-5.14 |
| 14 |
Morris, Player A (10)
|
NCGCY |
51.33 |
48.30 |
-3.03 |
| 15 |
Bernal, Nevaeh L (9)
|
NCLOY |
|
49.12 |
- |
| 16 |
Herlocker, Patterson L (9)
|
NCRACY |
52.80 |
50.20 |
-2.60 |
| 17 |
Monroe, Mia E (10)
|
NCNCAC |
1:00.99 |
50.74 |
-10.25 |
| 18 |
Drexel, Isabelle M (10)
|
NCSGSA |
50.86 |
52.70 |
1.84 |
| 19 |
Swinteck, Brynley C (9)
|
NCSGSA |
1:03.07 |
56.92 |
-6.15 |
| 20 |
Nelson, Zoe K (10)
|
NCSGSA |
1:00.99 |
56.99 |
-4.00 |
| 21 |
Combs, Annabelle P (9)
|
VACCA |
1:04.35 |
59.98 |
-4.37 |
| 22 |
Law, Casey E (10)
|
NCLOY |
|
1:01.14 |
- |
| 23 |
Delgado, Madison F (10)
|
NCNCAC |
1:00.99 |
1:02.79 |
1.80 |
|
Cao, Sophie Z (10)
|
NCLOY |
|
DQ |
- |
|
Raghunathan, Aarya V (10)
|
NCNCAC |
1:00.99 |
DQ |
- |
|
Espinosa, Madisyn G (9)
|
NCSGSA |
1:02.33 |
DQ |
- |
|
Hansing, Adasyn E (9)
|
NCSGSA |
|
DQ |
- |