| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wehe, Eden J (8)
|
NCGCY |
|
24.48 |
- |
| 2 |
Balfour, Genevieve J (8)
|
NCGCY |
27.14 |
25.83 |
-1.31 |
| 3 |
Longo, Gianna M (8)
|
NCNCAC |
30.99 |
25.84 |
-5.15 |
| 4 |
Auer, Theresa G (7)
|
NCGCY |
29.78 |
26.24 |
-3.54 |
| 5 |
Jacobs, Sophie G (8)
|
NCNCAC |
30.99 |
26.84 |
-4.15 |
| 6 |
Henderson, Brooke C (7)
|
NCGCY |
|
27.55 |
- |
| 7 |
Abernathy, Ella J (7)
|
NCGCY |
29.60 |
28.72 |
-0.88 |
| 8 |
Charles, Marie L (7)
|
NCNCAC |
35.68 |
29.17 |
-6.51 |
| 9 |
Mwanza, Florence E (7)
|
NCNCAC |
35.99 |
31.21 |
-4.78 |
| 10 |
Biswas, Anna A (7)
|
NCNCAC |
34.41 |
32.28 |
-2.13 |
| 11 |
Rak, Evelin L (8)
|
NCNCAC |
35.99 |
33.83 |
-2.16 |
| 12 |
Hodge, Julie A (7)
|
VACCA |
48.70 |
35.05 |
-13.65 |
|
Ballenger, Madeline K (8)
|
NCGCY |
32.95 |
DQ |
- |
|
Longo, Macaria M (6)
|
NCNCAC |
35.99 |
DQ |
- |
|
Raghunathan, Annika S (7)
|
NCNCAC |
35.99 |
DQ |
- |
|
Hawkins, Charlotte J (7)
|
NCRACY |
43.55 |
DQ |
- |