| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Adekoya, Oyinkansola O (10)
|
NCGCY |
30.80 |
30.48 |
-0.32 |
| 2 |
Collins, Daisy B (10)
|
NCNCAC |
31.72 |
30.67 |
-1.05 |
| 3 |
Liu, Reina R (9)
|
NCGCY |
32.57 |
31.55 |
-1.02 |
| 4 |
Brock, Yari E (9)
|
NCNCAC |
31.53 |
32.04 |
0.51 |
| 5 |
Wang, Sophie W (9)
|
NCGCY |
38.22 |
33.76 |
-4.46 |
| 6 |
Henderson, Catelyn G (10)
|
NCGCY |
34.32 |
34.17 |
-0.15 |
| 7 |
Morris, Player A (10)
|
NCGCY |
36.27 |
34.39 |
-1.88 |
| 8 |
Biswas, Emma A (10)
|
NCNCAC |
35.62 |
35.65 |
0.03 |
| 9 |
Testa, Madeline C (10)
|
NCSGSA |
40.05 |
35.81 |
-4.24 |
| 10 |
Bernal, Nevaeh L (9)
|
NCLOY |
|
36.10 |
- |
| 11 |
Buttar, Amira A (10)
|
NCLOY |
38.91 |
36.21 |
-2.70 |
| 12 |
Meadows, Emma F (10)
|
VACCA |
35.23 |
36.41 |
1.18 |
| 13 |
Monroe, Mia E (10)
|
NCNCAC |
50.99 |
36.91 |
-14.08 |
| 14 |
Kallepalli, Anya (10)
|
NCNCAC |
36.57 |
37.65 |
1.08 |
| 15 |
Palmer, Jaylin A (10)
|
NCGCY |
42.99 |
38.69 |
-4.30 |
| 16 |
Drexel, Isabelle M (10)
|
NCSGSA |
38.87 |
39.47 |
0.60 |
| 17 |
Ma, Runhui (10)
|
NCNCAC |
50.99 |
39.77 |
-11.22 |
| 18 |
Nelson, Zoe K (10)
|
NCSGSA |
40.77 |
40.76 |
-0.01 |
| 19 |
Herlocker, Patterson L (9)
|
NCRACY |
45.95 |
41.69 |
-4.26 |
| 20 |
Combs, Annabelle P (9)
|
VACCA |
43.75 |
43.57 |
-0.18 |
| 21 |
Law, Casey E (10)
|
NCLOY |
|
43.62 |
- |
| 22 |
Fullagar, Evie R (9)
|
NCSGSA |
|
44.10 |
- |
| 23 |
Shanks, Celia D (9)
|
VACCA |
51.23 |
44.43 |
-6.80 |
| 24 |
Delgado, Madison F (10)
|
NCNCAC |
50.99 |
44.98 |
-6.01 |
| 25 |
Espinosa, Madisyn G (9)
|
NCSGSA |
49.14 |
46.08 |
-3.06 |
| 26 |
Cao, Sophie Z (10)
|
NCLOY |
|
47.06 |
- |
| 27 |
Swinteck, Brynley C (9)
|
NCSGSA |
58.75 |
47.37 |
-11.38 |
| 28 |
Hansing, Adasyn E (9)
|
NCSGSA |
|
52.96 |
- |
| 29 |
Raghunathan, Aarya V (10)
|
NCNCAC |
59.85 |
56.51 |
-3.34 |