| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mu, Andrew L (17)
|
NCNCAC |
23.05 |
22.76 |
-0.29 |
| 2 |
Tars, Robert M (16)
|
NCGCY |
22.40 |
22.77 |
0.37 |
| 3 |
Williams, Camden H (16)
|
NCWA |
23.32 |
23.20 |
-0.12 |
| 4 |
Gatling III, Ladaniel (15)
|
NCGCY |
22.84 |
23.31 |
0.47 |
| 5 |
Jerger, Jacob A (16)
|
NCWA |
22.72 |
23.47 |
0.75 |
| 6 |
Brooks, Jason D (15)
|
NCGCY |
23.64 |
23.48 |
-0.16 |
| 7 |
Yeh, Alexander T (15)
|
NCLOY |
23.07 |
23.79 |
0.72 |
| 8 |
Esposito, William P (16)
|
NCGCY |
23.69 |
24.14 |
0.45 |
| 9 |
Lucktong, Samuel P (15)
|
NCLOY |
23.84 |
24.42 |
0.58 |
| 10 |
Yeh, Ryan T (16)
|
NCLOY |
24.03 |
24.43 |
0.40 |
| 11 |
Kachavos, Arman W (15)
|
NCNCAC |
25.47 |
24.54 |
-0.93 |
| 12 |
Muller, Nicholas A (17)
|
NCLOY |
24.60 |
24.76 |
0.16 |
| 13 |
Miroshnichenko, Alexander A (16)
|
NCGCY |
25.04 |
24.79 |
-0.25 |
| 13 |
Claborn, Nathan M (15)
|
NCLOY |
25.79 |
24.79 |
-1.00 |
| 15 |
Baker, Khari D (15)
|
NCNCAC |
24.89 |
25.08 |
0.19 |
| 16 |
Zhang, Ray (16)
|
NCGCY |
24.20 |
25.28 |
1.08 |
| 17 |
Rock, Owen G (17)
|
NCGCY |
24.60 |
25.38 |
0.78 |
| 18 |
Carsten, Nathaniel D (16)
|
NCLOY |
24.68 |
25.63 |
0.95 |
| 19 |
Nicholson, Graham T (16)
|
NCNCAC |
25.07 |
25.81 |
0.74 |
| 20 |
Vagner, Braxton S (16)
|
NCRACY |
|
26.62 |
- |
| 21 |
Conde, Anthony J (16)
|
NCLOY |
27.53 |
27.97 |
0.44 |
| 22 |
Cao, Benjamin M (15)
|
NCLOY |
28.00 |
27.98 |
-0.02 |
| 23 |
Beck, Zachary D (16)
|
NCLOY |
27.88 |
28.43 |
0.55 |
| 24 |
Krafft, Jordan L (15)
|
NCWA |
|
28.62 |
- |
|
Latorre, Daniel S (16)
|
NCLOY |
29.02 |
NS |
- |