| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Balfour, Genevieve J (8)
|
NCGCY |
24.30 |
23.89 |
-0.41 |
| 2 |
Mwanza, Florence E (7)
|
NCNCAC |
30.99 |
24.67 |
-6.32 |
| 3 |
Ballenger, Madeline K (8)
|
NCGCY |
25.36 |
25.38 |
0.02 |
| 4 |
Henderson, Brooke C (7)
|
NCGCY |
25.34 |
25.68 |
0.34 |
| 5 |
Auer, Theresa G (7)
|
NCGCY |
25.56 |
26.11 |
0.55 |
| 6 |
Abernathy, Ella J (7)
|
NCGCY |
27.73 |
26.97 |
-0.76 |
| 7 |
Wang, Ellen (7)
|
NCGCY |
|
29.24 |
- |
| 8 |
Hawkins, Charlotte J (7)
|
NCRACY |
30.74 |
29.81 |
-0.93 |
| 9 |
Longo, Macaria M (6)
|
NCNCAC |
30.99 |
33.79 |
2.80 |
| 10 |
Raghunathan, Annika S (7)
|
NCNCAC |
30.99 |
36.79 |
5.80 |
|
Olalere, Alexandra R (7)
|
NCGCY |
23.19 |
DQ |
- |
|
Wehe, Eden J (8)
|
NCGCY |
|
DQ |
- |
|
Rak, Evelin L (8)
|
NCNCAC |
30.99 |
DQ |
- |