| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Liu, Reina R (9)
|
NCGCY |
36.52 |
35.13 |
-1.39 |
| 2 |
Collins, Daisy B (10)
|
NCNCAC |
34.17 |
35.52 |
1.35 |
| 3 |
Biswas, Emma A (10)
|
NCNCAC |
40.98 |
38.31 |
-2.67 |
| 4 |
Henderson, Catelyn G (10)
|
NCGCY |
36.56 |
38.91 |
2.35 |
| 5 |
Morris, Player A (10)
|
NCGCY |
40.11 |
39.48 |
-0.63 |
| 6 |
Monroe, Mia E (10)
|
NCNCAC |
1:00.99 |
41.80 |
-19.19 |
| 7 |
Brock, Yari E (9)
|
NCNCAC |
39.35 |
42.65 |
3.30 |
| 8 |
Buttar, Amira A (10)
|
NCLOY |
44.53 |
42.84 |
-1.69 |
| 9 |
DeLoreto, Dorothea A (9)
|
NCSGSA |
44.08 |
42.92 |
-1.16 |
| 10 |
Bernal, Nevaeh L (9)
|
NCLOY |
|
43.16 |
- |
| 11 |
Hicks, Jozi E (10)
|
NCUN |
42.85 |
43.30 |
0.45 |
| 12 |
Kallepalli, Anya (10)
|
NCNCAC |
41.43 |
43.41 |
1.98 |
| 13 |
Ma, Runhui (10)
|
NCNCAC |
1:00.99 |
46.01 |
-14.98 |
| 14 |
Drexel, Isabelle M (10)
|
NCSGSA |
44.66 |
48.72 |
4.06 |
| 15 |
Nelson, Zoe K (10)
|
NCSGSA |
50.32 |
53.91 |
3.59 |
| 16 |
Shanks, Celia D (9)
|
VACCA |
1:05.14 |
54.01 |
-11.13 |
| 17 |
Fullagar, Evie R (9)
|
NCSGSA |
|
55.80 |
- |
| 18 |
Cao, Sophie Z (10)
|
NCLOY |
|
56.97 |
- |
| 19 |
Law, Casey E (10)
|
NCLOY |
|
57.23 |
- |
|
Delgado, Madison F (10)
|
NCNCAC |
55.99 |
DQ |
- |
|
Swinteck, Brynley C (9)
|
NCSGSA |
|
DQ |
- |
|
Raghunathan, Aarya V (10)
|
NCNCAC |
1:00.99 |
NS |
- |