| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Olalere, Anderson J (10)
|
NCGCY |
31.43 |
32.72 |
1.29 |
| 2 |
Wehe, Isaiah J (10)
|
NCGCY |
|
33.69 |
- |
| 3 |
Gaylord, Thomas J (9)
|
NCGCY |
|
38.29 |
- |
| 4 |
Halpern, Maximillian N (10)
|
NCNCAC |
40.00 |
39.36 |
-0.64 |
| 5 |
Wulber, Fletcher D (10)
|
NCNCAC |
40.87 |
39.98 |
-0.89 |
| 6 |
Cooper, Henry D (10)
|
NCGCY |
|
42.41 |
- |
| 7 |
Parker, Grant E (10)
|
NCLOY |
53.63 |
42.72 |
-10.91 |
| 8 |
Allen, Benjamin O (10)
|
NCLOY |
|
43.05 |
- |
| 9 |
Austria, Elijah S (9)
|
NCGCY |
51.74 |
44.33 |
-7.41 |
| 10 |
Mitler, Charles G (9)
|
NCNCAC |
1:00.99 |
44.68 |
-16.31 |
| 11 |
Ray, Lucas J (10)
|
NCGCY |
49.41 |
45.09 |
-4.32 |
| 12 |
Boehlke, Noah G (9)
|
NCNCAC |
49.43 |
46.53 |
-2.90 |
| 13 |
Meck, Wyatt D (9)
|
NCSGSA |
45.65 |
46.82 |
1.17 |
| 14 |
Murray, Cody J (10)
|
NCLOY |
52.18 |
48.45 |
-3.73 |
| 14 |
Coltrane, Chasen A (10)
|
NCSGSA |
42.61 |
48.45 |
5.84 |
| 16 |
King, Logan S (9)
|
NCLOY |
|
51.17 |
- |
| 17 |
He, Bryan T (9)
|
NCGCY |
|
52.03 |
- |
| 18 |
Wolk, Max A (9)
|
NCNCAC |
57.05 |
53.07 |
-3.98 |
| 19 |
Eggleston, Cannon S (9)
|
NCSGSA |
1:00.90 |
53.53 |
-7.37 |
| 20 |
Podgoreanu, Joseph O (10)
|
NCNCAC |
56.24 |
55.07 |
-1.17 |
| 21 |
Maiorana, Aaron T (9)
|
NCNCAC |
56.01 |
1:03.00 |
6.99 |
| 22 |
Thompson, Charles M (9)
|
NCSGSA |
|
1:14.13 |
- |
|
Gatling, Carl F (9)
|
NCGCY |
|
DQ |
- |
|
Zhang, Ximin S (9)
|
NCGCY |
|
DQ |
- |