| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jerger, Jacob A (16)
|
NCWA |
34.61 |
25.70 |
-8.91 |
| 2 |
Miroshnichenko, Alexander A (16)
|
NCGCY |
26.80 |
25.79 |
-1.01 |
| 3 |
Williams, Camden H (16)
|
NCWA |
28.86 |
25.96 |
-2.90 |
| 4 |
Yeh, Alexander T (15)
|
NCLOY |
28.73 |
26.32 |
-2.41 |
| 5 |
Yeh, Ryan T (16)
|
NCLOY |
27.99 |
26.94 |
-1.05 |
| 6 |
Claborn, Nathan M (15)
|
NCLOY |
29.31 |
27.11 |
-2.20 |
| 7 |
Esposito, William P (16)
|
NCGCY |
26.93 |
27.85 |
0.92 |
| 8 |
Lucktong, Samuel P (15)
|
NCLOY |
28.99 |
28.53 |
-0.46 |
| 9 |
Rock, Owen G (17)
|
NCGCY |
28.71 |
28.54 |
-0.17 |
| 10 |
Newman, Aaron M (16)
|
NCGCY |
|
28.66 |
- |
| 11 |
Muller, Nicholas A (17)
|
NCLOY |
31.44 |
28.70 |
-2.74 |
| 12 |
Carsten, Nathaniel D (16)
|
NCLOY |
29.30 |
29.79 |
0.49 |
| 13 |
Conde, Anthony J (16)
|
NCLOY |
32.19 |
30.52 |
-1.67 |
| 14 |
Krafft, Jordan L (15)
|
NCWA |
|
31.68 |
- |
| 15 |
Cao, Benjamin M (15)
|
NCLOY |
38.45 |
31.83 |
-6.62 |
| 16 |
Beck, Zachary D (16)
|
NCLOY |
36.21 |
34.06 |
-2.15 |
|
Latorre, Daniel S (16)
|
NCLOY |
|
NS |
- |