| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Isabel H (11)
|
NCNCAC |
57.64 |
57.11 |
-0.53 |
| 2 |
Collins, Mackenzie K (11)
|
NCNCAC |
1:03.22 |
1:01.94 |
-1.28 |
| 3 |
Spurlock, Avery M (11)
|
VACCA |
1:06.36 |
1:03.24 |
-3.12 |
| 4 |
Hodge, Anna M (12)
|
VACCA |
1:02.96 |
1:03.43 |
0.47 |
| 5 |
Giovanello, Aurora J (12)
|
NCNCAC |
1:04.65 |
1:04.07 |
-0.58 |
| 6 |
McGee, Sophia G (12)
|
NCRACY |
1:07.72 |
1:08.70 |
0.98 |
| 7 |
Kim, Kenzie A (11)
|
NCNCAC |
1:08.43 |
1:09.09 |
0.66 |
| 8 |
Conforti-Johnson, Sophia K (12)
|
VACCA |
|
1:09.53 |
- |
| 9 |
Burton, Chloe G (11)
|
NCNCAC |
1:16.41 |
1:13.71 |
-2.70 |
| 10 |
Taub, Anna J (12)
|
NCNCAC |
1:17.26 |
1:16.76 |
-0.50 |
| 11 |
Waters, Zoey L (11)
|
NCRACY |
1:23.01 |
1:19.42 |
-3.59 |
| 12 |
Li, Joie N (11)
|
NCNCAC |
1:25.09 |
1:20.71 |
-4.38 |
| 13 |
Ballenger, Emma G (11)
|
NCGCY |
1:26.99 |
1:23.27 |
-3.72 |
| 14 |
Kayande, Anya S (11)
|
NCGCY |
1:36.16 |
1:23.54 |
-12.62 |
| 15 |
Jernigan, Jordyn M (11)
|
NCRACY |
1:24.62 |
1:25.65 |
1.03 |