| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Collins, Daisy B (10)
|
NCNCAC |
37.90 |
37.46 |
-0.44 |
| 2 |
Liu, Reina R (9)
|
NCGCY |
40.62 |
38.22 |
-2.40 |
| 3 |
Adekoya, Oyinkansola O (10)
|
NCGCY |
40.25 |
38.40 |
-1.85 |
| 4 |
Henderson, Catelyn G (10)
|
NCGCY |
38.59 |
40.48 |
1.89 |
| 5 |
Wang, Sophie W (9)
|
NCGCY |
49.07 |
40.72 |
-8.35 |
| 6 |
Testa, Madeline C (10)
|
NCSGSA |
46.50 |
41.41 |
-5.09 |
| 7 |
Monroe, Mia E (10)
|
NCNCAC |
50.99 |
42.64 |
-8.35 |
| 8 |
Bernal, Nevaeh L (9)
|
NCLOY |
|
44.42 |
- |
| 9 |
Ma, Runhui (10)
|
NCNCAC |
50.99 |
44.72 |
-6.27 |
| 10 |
Biswas, Emma A (10)
|
NCNCAC |
44.33 |
44.94 |
0.61 |
| 11 |
Meadows, Emma F (10)
|
VACCA |
45.52 |
46.45 |
0.93 |
| 12 |
DeLoreto, Dorothea A (9)
|
NCSGSA |
44.02 |
46.84 |
2.82 |
| 13 |
Herlocker, Patterson L (9)
|
NCRACY |
46.12 |
46.92 |
0.80 |
| 14 |
Kallepalli, Anya (10)
|
NCNCAC |
45.08 |
47.45 |
2.37 |
| 15 |
Combs, Annabelle P (9)
|
VACCA |
52.12 |
49.21 |
-2.91 |
| 16 |
Drexel, Isabelle M (10)
|
NCSGSA |
49.11 |
49.26 |
0.15 |
| 17 |
Nelson, Zoe K (10)
|
NCSGSA |
49.13 |
50.13 |
1.00 |
| 18 |
Cao, Sophie Z (10)
|
NCLOY |
|
51.17 |
- |
| 19 |
Swinteck, Brynley C (9)
|
NCSGSA |
1:03.14 |
51.22 |
-11.92 |
| 20 |
Fullagar, Evie R (9)
|
NCSGSA |
|
51.87 |
- |
| 21 |
Hansing, Adasyn E (9)
|
NCSGSA |
|
53.03 |
- |
| 22 |
Law, Casey E (10)
|
NCLOY |
|
53.47 |
- |
| 23 |
Shanks, Celia D (9)
|
VACCA |
52.07 |
54.70 |
2.63 |
| 24 |
Raghunathan, Aarya V (10)
|
NCNCAC |
1:03.60 |
55.47 |
-8.13 |
|
Delgado, Madison F (10)
|
NCNCAC |
50.99 |
DQ |
- |
|
Brock, Yari E (9)
|
NCNCAC |
36.57 |
NS |
- |
|
Espinosa, Madisyn G (9)
|
NCSGSA |
|
NS |
- |