| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vargas, Hanna S (10)
|
NCTYDE |
36.29 |
34.66 |
-1.63 |
| 2 |
Henderson, Catelyn G (10)
|
NCGCY |
38.59 |
37.60 |
-0.99 |
| 3 |
Liu, Reina R (9)
|
NCGCY |
38.22 |
38.48 |
0.26 |
| 4 |
Adekoya, Oyinkansola O (10)
|
NCGCY |
38.40 |
39.08 |
0.68 |
| 5 |
Wang, Sophie W (9)
|
NCGCY |
40.72 |
42.81 |
2.09 |
| 6 |
Wheeler, Alyssa N (10)
|
NCTYDE |
44.39 |
43.35 |
-1.04 |
| 7 |
Drexel, Isabelle M (10)
|
NCSGSA |
46.80 |
43.42 |
-3.38 |
| 8 |
Abernathy, Ella J (8)
|
NCGCY |
|
51.51 |
- |
| 9 |
Balfour, Genevieve J (8)
|
NCGCY |
52.68 |
52.12 |
-0.56 |
| 10 |
Bunton, Cora R (6)
|
NCSGSA |
|
1:01.14 |
- |
|
Bunton, Lamia P (8)
|
NCSGSA |
|
DQ |
- |