| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barrick, Riley D (8)
|
NCSAC |
43.51 |
41.46 |
-2.05 |
| 2 |
Millsaps, Katherine G (7)
|
NCCVAC |
45.86 |
41.78 |
-4.08 |
| 3 |
McIntyre, Elizabeth G (8)
|
NCATOM |
45.93 |
45.54 |
-0.39 |
| 4 |
Huffman, Madison L (8)
|
NCCVAC |
58.20 |
53.46 |
-4.74 |
| 5 |
Bartlett, Veronica D (8)
|
NCCVAC |
59.20 |
53.70 |
-5.50 |
| 6 |
Stancil, Ellie G (8)
|
NCGG |
1:01.62 |
56.50 |
-5.12 |
| 7 |
Beltran-Trejo, Fernanda (7)
|
NCCVAC |
1:04.90 |
59.00 |
-5.90 |
| 8 |
Royall, Payton E (8)
|
NCGG |
|
1:00.49 |
- |
| 9 |
Hernandez, Diana (7)
|
NCCVAC |
|
1:04.97 |
- |
| 10 |
Sheets, Brooke (8)
|
NCAQTC |
|
1:06.00 |
- |
| 11 |
McCraw, Reese M (6)
|
NCRCST |
|
1:09.19 |
- |
| 12 |
Yates, Cayla L (8)
|
NCSAC |
|
1:10.39 |
- |
| 13 |
Pintuff, Maci L (5)
|
NCRCST |
|
1:17.21 |
- |
| 14 |
Millsaps, Caroline M (5)
|
NCCVAC |
|
1:46.60 |
- |