| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schneider, Taia M (10)
|
NCRCST |
48.41 |
45.16 |
-3.25 |
| 2 |
McNeal, Ella J (10)
|
NCGG |
47.08 |
45.45 |
-1.63 |
| 3 |
Leazer, Audrey H (10)
|
NCGG |
51.42 |
47.65 |
-3.77 |
| 4 |
Robinson, Lillian B (10)
|
NCGG |
56.44 |
49.18 |
-7.26 |
| 5 |
Phillips, Elizabeth M (10)
|
NCGG |
1:16.70 |
53.27 |
-23.43 |
| 6 |
Teague, Lauren M (10)
|
NCNMA |
|
57.61 |
- |
| 7 |
Willer, Avery L (9)
|
NCGG |
1:05.47 |
58.68 |
-6.79 |
| 8 |
Bullins, Blakeney E (9)
|
NCATOM |
57.52 |
58.74 |
1.22 |
| 9 |
Hollingsworth, Makenzie L (9)
|
NCSAC |
|
59.71 |
- |
| 10 |
Walker, Alyssa D (10)
|
NCGG |
|
59.76 |
- |
| 11 |
Hall, Paige D (9)
|
NCATOM |
1:00.52 |
59.79 |
-0.73 |
| 12 |
Hennessy, Ella J (9)
|
NCATOM |
1:02.79 |
1:01.50 |
-1.29 |
| 13 |
Suber, Eva C (10)
|
NCGG |
1:19.29 |
1:05.28 |
-14.01 |
| 14 |
Jones, Paige B (9)
|
NCGG |
1:10.02 |
1:06.18 |
-3.84 |
| 15 |
Hill, Elise S (9)
|
NCNMA |
1:03.92 |
1:08.82 |
4.90 |
| 16 |
Yates, Carleigh G (9)
|
NCSAC |
|
1:23.43 |
- |
|
Casiano, Zoe (9)
|
NCCVAC |
|
DQ |
- |