| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Edwards, Jonathan P (14)
|
NCHPSC |
23.39 |
23.87 |
0.48 |
| 2 |
Cridlebaugh, Rowan P (13)
|
NCHPSC |
25.01 |
25.18 |
0.17 |
| 3 |
Clark, Quintin S (13)
|
NCSTAR |
25.67 |
25.87 |
0.20 |
| 4 |
Smith, Braeden G (14)
|
NCHPSC |
27.78 |
27.13 |
-0.65 |
| 5 |
Kafitz, Christopher S (14)
|
NCRACY |
27.98 |
28.10 |
0.12 |
| 6 |
Perez-Hurtado, Gerardo (13)
|
NCMSA |
30.42 |
30.03 |
-0.39 |
| 7 |
Greene, Mark L (14)
|
NCMSA |
31.12 |
30.81 |
-0.31 |
| 8 |
Xu, David K (14)
|
NCMSA |
33.49 |
32.10 |
-1.39 |
| 9 |
Matallana, Adrian V (13)
|
NCFISH |
44.66 |
40.67 |
-3.99 |
|
Jones, Richard J (14)
|
NCMSA |
|
DQ |
- |