| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bradshaw, Lane M (14)
|
NCUN-S |
22.80 |
22.52 |
-0.28 |
| 2 |
Solvason, Connor C (14)
|
NCSAIL |
24.04 |
23.96 |
-0.08 |
| 3 |
Spinner, Wiley J (13)
|
NCMSA |
23.73 |
24.09 |
0.36 |
| 4 |
Sypolt, Owen N (14)
|
NCSAIL |
23.78 |
24.28 |
0.50 |
| 5 |
Taylor, Brendan W (13)
|
NCMSA |
24.29 |
24.59 |
0.30 |
| 6 |
Engel, Connor J (14)
|
NCTEAM |
24.70 |
24.92 |
0.22 |
| 7 |
Vadapalli, Bhargav S (14)
|
NCSAIL |
25.19 |
25.07 |
-0.12 |
| 8 |
Losh, Christian I (14)
|
NCMSA |
25.68 |
25.10 |
-0.58 |
| 9 |
Peterson, Caleb A (13)
|
NCMSA |
24.35 |
25.12 |
0.77 |
| 10 |
Xie, David (13)
|
NCMSA |
26.09 |
25.30 |
-0.79 |
| 11 |
Berry, Nolan J (14)
|
NCSAIL |
24.65 |
25.53 |
0.88 |
| 12 |
Scott, Luke M (13)
|
NCMSA |
26.19 |
25.68 |
-0.51 |
| 13 |
James, Alexander M (14)
|
NCMSA |
26.32 |
25.77 |
-0.55 |
| 14 |
Luong, Preston Y (14)
|
NCSAIL |
26.28 |
25.86 |
-0.42 |
| 15 |
Fletcher, Alden N (14)
|
NCTEAM |
25.40 |
26.03 |
0.63 |
| 16 |
Shivers, Payton P (14)
|
NCMSA |
29.99 |
26.26 |
-3.73 |
| 17 |
Winardi, Tobias T (13)
|
NCMSA |
26.47 |
26.35 |
-0.12 |
| 18 |
Simmons, Nelson A (13)
|
NCSAIL |
25.72 |
26.37 |
0.65 |
| 19 |
Tomcho, Briar C (14)
|
NCTEAM |
26.94 |
27.63 |
0.69 |
| 20 |
Perry, Dylan J (14)
|
NCSAIL |
30.29 |
28.18 |
-2.11 |
| 21 |
Burk, Layton A (14)
|
NCMSA |
31.74 |
28.29 |
-3.45 |
| 22 |
Dolan, Luke M (13)
|
NCSAIL |
29.10 |
28.57 |
-0.53 |
| 23 |
Pillai, Nik P (13)
|
NCMSA |
29.22 |
28.87 |
-0.35 |
| 24 |
Ashri, Nikhil (13)
|
NCMSA |
29.97 |
29.28 |
-0.69 |
| 25 |
Clabault, Alex E (14)
|
NCMSA |
28.72 |
29.57 |
0.85 |
| 26 |
Thomas, Robert P (13)
|
NCTEAM |
31.75 |
30.45 |
-1.30 |
|
Schmidt, Andrew R (14)
|
NCSAIL |
26.80 |
NS |
- |
|
Schiff, Britton A (13)
|
NCSAIL |
|
NS |
- |
|
Schiff, Braydon A (13)
|
NCSAIL |
|
NS |
- |
|
Dodla, Navneet R (13)
|
NCSAIL |
37.99 |
NS |
- |
|
Rosenbloom, Joshua W (14)
|
NCMSA |
25.44 |
NS |
- |