| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ramseur, Riley G (10)
|
NCSFSC |
31.47 |
31.40 |
-0.07 |
| 2 |
McMasters, Sophie R (10)
|
NCSFSC |
33.15 |
34.13 |
0.98 |
| 3 |
Gill, Anna K (10)
|
NCSFSC |
33.47 |
34.67 |
1.20 |
| 4 |
Holmes, Kylie Grace L (8)
|
NCSFSC |
37.66 |
37.83 |
0.17 |
| 5 |
Jones, Eloise E (10)
|
NCSFSC |
39.49 |
38.67 |
-0.82 |
| 6 |
Binder, Lilian J (10)
|
NCSFSC |
40.54 |
40.83 |
0.29 |
| 7 |
Ault, Serenity E (10)
|
NCSFSC |
41.77 |
42.42 |
0.65 |
| 8 |
Maley, Samantha C (10)
|
NCSFSC |
48.20 |
47.54 |
-0.66 |
| 9 |
Tabori, Emily M (8)
|
NCSFSC |
|
47.82 |
- |
| 10 |
Smith, Molly E (8)
|
NCSFSC |
|
48.03 |
- |
| 11 |
Coyne, Morgan I (9)
|
NCSTAR |
58.62 |
50.16 |
-8.46 |
| 12 |
Conklin, Highland S (10)
|
NCSFSC |
50.06 |
50.44 |
0.38 |
| 13 |
Sydell, Sidney G (10)
|
NCSFSC |
|
50.49 |
- |
| 14 |
Bumgarner, Carolina F (9)
|
NCSTAR |
57.42 |
53.83 |
-3.59 |
| 15 |
Smith, Emma R (10)
|
NCSFSC |
1:02.91 |
1:01.39 |
-1.52 |
|
Sakalouskaya, Valerie G (9)
|
NCSFSC |
|
NS |
- |