| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ramseur, Riley G (10)
|
NCSFSC |
38.17 |
37.53 |
-0.64 |
| 2 |
Gill, Anna K (10)
|
NCSFSC |
45.08 |
41.86 |
-3.22 |
| 3 |
Jones, Eloise E (10)
|
NCSFSC |
41.96 |
41.99 |
0.03 |
| 4 |
McMasters, Sophie R (10)
|
NCSFSC |
44.79 |
43.69 |
-1.10 |
| 5 |
Sakalouskaya, Valerie G (9)
|
NCSFSC |
|
45.42 |
- |
| 6 |
Ault, Serenity E (10)
|
NCSFSC |
46.76 |
45.70 |
-1.06 |
| 7 |
Binder, Lilian J (10)
|
NCSFSC |
54.40 |
49.53 |
-4.87 |
| 8 |
Holmes, Kylie Grace L (8)
|
NCSFSC |
51.66 |
50.48 |
-1.18 |
| 9 |
Tabori, Emily M (8)
|
NCSFSC |
51.57 |
52.85 |
1.28 |
| 10 |
Maley, Samantha C (10)
|
NCSFSC |
50.47 |
53.17 |
2.70 |
| 11 |
Sydell, Sidney G (10)
|
NCSFSC |
52.05 |
53.53 |
1.48 |
| 12 |
Bumgarner, Carolina F (9)
|
NCSTAR |
54.31 |
55.36 |
1.05 |
| 13 |
Coyne, Morgan I (9)
|
NCSTAR |
1:04.94 |
59.28 |
-5.66 |
| 14 |
Smith, Molly E (8)
|
NCSFSC |
1:00.35 |
1:00.13 |
-0.22 |
| 15 |
Smith, Emma R (10)
|
NCSFSC |
58.66 |
1:01.45 |
2.79 |
| 16 |
Conklin, Highland S (10)
|
NCSFSC |
1:02.76 |
1:03.87 |
1.11 |
| 17 |
Colleran, Aoibhin G (7)
|
NCSTAR |
|
1:09.71 |
- |