| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Valliere, Tucker L (14)
|
NCSMAC |
26.48 |
26.80 |
0.32 |
| 2 |
Gonzales, Luke (13)
|
NCSMAC |
28.05 |
28.38 |
0.33 |
| 3 |
Lunsik, Dylan J (14)
|
NCSMAC |
28.61 |
28.50 |
-0.11 |
| 4 |
Sankey-Taranto, Sage R (16)
|
NCSMAC |
28.59 |
28.53 |
-0.06 |
| 5 |
Fishman, Isaac B (15)
|
NCSMAC |
30.13 |
29.09 |
-1.04 |
| 6 |
Mina, Luke A (16)
|
NCSMAC |
28.77 |
30.22 |
1.45 |
| 7 |
Aleman, Liam D (13)
|
NCSMAC |
29.25 |
30.96 |
1.71 |
| 8 |
Allen, Jacob E (17)
|
NCSMAC |
29.20 |
32.40 |
3.20 |
| 9 |
Shainberg, David T (19)
|
NCSMAC |
29.93 |
32.72 |
2.79 |
| 10 |
Galindo-Gomez, Edwen J (13)
|
NCSMAC |
32.47 |
34.16 |
1.69 |
| 11 |
Moran, Martin M (13)
|
NCSMAC |
31.66 |
37.56 |
5.90 |
| 12 |
Serenius, Max J (9)
|
NCSMAC |
40.23 |
41.44 |
1.21 |