| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gendzel, Maya C (14)
|
NCMAC |
23.68 |
24.15 |
0.47 |
| 2 |
Rhodes, Olivia A (16)
|
NCMAC |
24.63 |
24.22 |
-0.41 |
| 3 |
Francis, Sophia W (18)
|
NCMAC |
23.78 |
24.28 |
0.50 |
| 4 |
Steelhammer, Gracie B (17)
|
NCMAC |
24.11 |
24.69 |
0.58 |
| 5 |
Barnett, Claudia J (19)
|
NCMAC |
24.29 |
24.75 |
0.46 |
| 6 |
Baumgartner, Hannah G (15)
|
NCMAC |
25.16 |
25.00 |
-0.16 |
| 7 |
Wozny, Rhiannon M (18)
|
NCMAC |
25.18 |
25.14 |
-0.04 |
| 8 |
Fleury, Sarah K (15)
|
NCMAC |
24.71 |
25.30 |
0.59 |
| 9 |
Murray, Avery E (15)
|
NCMAC |
25.38 |
25.37 |
-0.01 |
| 10 |
Siemasko, Lily M (18)
|
NCMAC |
24.55 |
25.43 |
0.88 |
| 11 |
Lyons, Claire M (15)
|
NCMAC |
25.31 |
25.66 |
0.35 |
| 12 |
Gehrig, Julia J (14)
|
NCMAC |
26.13 |
26.10 |
-0.03 |
| 13 |
Claudy, Madison A (17)
|
NCMAC |
25.72 |
26.17 |
0.45 |
| 14 |
Lang, Katherine R (15)
|
NCMAC |
26.16 |
26.33 |
0.17 |
| 15 |
Brewer, Ashtyn B (11)
|
NCMAC |
26.80 |
26.71 |
-0.09 |
| 16 |
Powell, Olivia V (16)
|
NCMAC |
26.78 |
26.74 |
-0.04 |
| 17 |
McCarthy, Grace R (10)
|
NCMAC |
30.33 |
29.43 |
-0.90 |
| 18 |
Szostek, Alexis J (10)
|
NCMAC |
31.47 |
30.54 |
-0.93 |
| 19 |
Hollar, Caylee A (10)
|
NCMAC |
33.04 |
30.83 |
-2.21 |