| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Saxena, Naisha (11)
|
NCTAC |
34.47 |
35.58 |
1.11 |
| 2 |
Manners, Ava J (11)
|
NCGFSC |
34.39 |
36.16 |
1.77 |
| 3 |
Heise, Ashlin D (14)
|
NCLTNC |
42.02 |
39.14 |
-2.88 |
| 4 |
Ashford, Madeleine S (11)
|
NCTAC |
41.01 |
39.20 |
-1.81 |
| 5 |
Flanagan, Madeleine G (12)
|
NCNEAL |
42.93 |
39.70 |
-3.23 |
| 6 |
Vechalapu, Nithi (12)
|
NCTAC |
48.00 |
42.53 |
-5.47 |
| 7 |
Honeycutt, Caroline H (11)
|
NCVACB |
55.44 |
49.23 |
-6.21 |
| 8 |
Thanumalayan, Suchitra N (11)
|
NCTAC |
48.00 |
51.31 |
3.31 |
| 9 |
Menon, Gouri A (11)
|
NCTAC |
|
56.38 |
- |
| 10 |
Liu, Mia J (13)
|
NCTAC |
|
1:00.27 |
- |
| 11 |
Trout, Madeline M (11)
|
NCTAC |
|
1:10.26 |
- |
| 12 |
Chakkravarthy, Cynthia B (11)
|
NCTAC |
|
1:19.01 |
- |
|
Allu, Sahasra G (11)
|
NCTAC |
|
DQ |
- |