| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sutter, Olivia C (17)
|
NCWNCY |
33.44 |
32.44 |
-1.00 |
| 2 |
Ally, Emily H (16)
|
NCUN |
29.27 |
32.68 |
3.41 |
| 3 |
Taylor, Kayana R (15)
|
NCYSST |
31.60 |
33.15 |
1.55 |
| 4 |
Kilpatrick, Ava S (15)
|
NCWNCY |
35.35 |
33.44 |
-1.91 |
| 5 |
Turcanu, Nina C (17)
|
NCYSST |
31.77 |
33.69 |
1.92 |
| 6 |
Hubbard, Ashley C (15)
|
SCYCSC |
33.28 |
34.06 |
0.78 |
| 7 |
Hernandez-Pena, Valeria (15)
|
NCYSST |
34.25 |
34.64 |
0.39 |
| 8 |
McGuire, Grace M (16)
|
NCRACY |
37.29 |
35.01 |
-2.28 |
| 9 |
Hixson, Kaelyn R (18)
|
NCWNCY |
39.12 |
37.97 |
-1.15 |
| 12 |
Allen, Emily P (15)
|
SCNYST |
|
39.29 |
- |
| 13 |
Kaczynski, Abigail E (15)
|
SCYCSC |
39.86 |
39.91 |
0.05 |
| 14 |
Brake, Hannah M (16)
|
NCWNCY |
|
41.00 |
- |
| 15 |
Hart, Savannah R (15)
|
NCRACY |
|
42.61 |
- |
| 16 |
Schreiber, Kaitlyn P (15)
|
SCYCSC |
43.31 |
44.58 |
1.27 |
| 17 |
Culp, Brenna E (17)
|
NCRACY |
39.90 |
44.61 |
4.71 |
|
Reeder, Grace C (18)
|
NCWNCY |
30.97 |
NS |
- |
|
Williams, Averi V (17)
|
NCWNCY |
|
NS |
- |