| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dant, Ross M (18)
|
NCYSST |
25.96 |
26.19 |
0.23 |
| 2 |
Thompson, Jakob E (17)
|
SCYCSC |
26.53 |
26.28 |
-0.25 |
| 3 |
McKenzie, Adam A (27)
|
SCAAYM |
25.30 |
26.63 |
1.33 |
| 4 |
Maximenya, Daniel C (15)
|
SCAAYM |
27.07 |
27.03 |
-0.04 |
| 5 |
James, Ben A (16)
|
VASFY |
25.95 |
27.10 |
1.15 |
| 6 |
Kolomiyets, Israel Z (17)
|
NCWNCY |
26.93 |
27.32 |
0.39 |
| 7 |
McCosh, Ethan A (16)
|
NCYSST |
27.60 |
27.49 |
-0.11 |
| 8 |
DeWalt, Logan C (16)
|
VASFY |
26.70 |
27.64 |
0.94 |
| 9 |
Hutchinson, Matthew S (17)
|
NCYSST |
27.97 |
27.76 |
-0.21 |
| 10 |
Macias-Martinez, Jose M (15)
|
SCAAYM |
28.58 |
27.93 |
-0.65 |
| 12 |
Fleenor, Boone M (15)
|
VASFY |
25.99 |
28.14 |
2.15 |
| 13 |
Howden, Frank B (15)
|
NCWNCY |
30.16 |
28.49 |
-1.67 |
| 14 |
King, Christopher H (15)
|
VASFY |
28.43 |
28.66 |
0.23 |
| 16 |
Brown, Daniel T (16)
|
SCYCSC |
27.49 |
28.96 |
1.47 |
| 17 |
Webb, William J (17)
|
SCYCSC |
30.27 |
29.07 |
-1.20 |
| 18 |
Parmenter, Carson J (15)
|
NCYSST |
30.32 |
29.56 |
-0.76 |
| 19 |
Kroi, Ernest G (18)
|
NCWNCY |
30.95 |
29.61 |
-1.34 |
| 21 |
MacDermott, Connor E (15)
|
NCRACY |
32.49 |
29.76 |
-2.73 |
| 22 |
Johnson, Aj (15)
|
NCWNCY |
27.60 |
29.94 |
2.34 |
| 23 |
Miles, James C (17)
|
VASFY |
29.17 |
30.46 |
1.29 |
| 24 |
Russell, Brayden S (15)
|
SCYCSC |
31.82 |
30.54 |
-1.28 |
| 26 |
MacDermott, Maddux D (16)
|
NCRACY |
30.35 |
31.80 |
1.45 |
| 27 |
Haas, Dylan R (17)
|
NCYSST |
30.28 |
31.98 |
1.70 |
| 28 |
Jackson, Jacob L (17)
|
NCRACY |
32.41 |
32.17 |
-0.24 |