| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Goldblum, McKaley Q (17)
|
NCWOW |
5:02.00 |
5:06.40 |
4.40 |
| 2 |
Furbay, Caroline D (13)
|
NCWOW |
5:39.12 |
5:44.33 |
5.21 |
| 3 |
Bishop, Avery M (15)
|
NCWOW |
5:57.23 |
5:45.86 |
-11.37 |
| 4 |
Forbes, Abigail R (13)
|
NCNSEA |
5:45.97 |
5:46.77 |
0.80 |
| 5 |
Brook, Annica S (13)
|
NCHCAC |
6:05.00 |
5:54.34 |
-10.66 |
| 6 |
Neathery, Peyton E (14)
|
NCWOW |
5:53.83 |
5:56.13 |
2.30 |
| 7 |
Weigand, Hailey J (13)
|
NCWOW |
6:15.05 |
6:01.97 |
-13.08 |
| 8 |
George, Ruby M (14)
|
NCHCAC |
6:01.00 |
6:04.35 |
3.35 |
| 9 |
Camacho, Eponine B (14)
|
NCNSEA |
6:07.53 |
6:06.87 |
-0.66 |
| 10 |
Jacocks, Joy Ruth M (15)
|
NCCFAC |
6:27.97 |
6:06.99 |
-20.98 |
| 11 |
Brown, Olivia C (13)
|
NCCFAC |
|
6:11.69 |
- |
| 12 |
Eder, Cadence S (13)
|
NCNSEA |
6:06.65 |
6:13.10 |
6.45 |
| 13 |
Hubard, Lucinda R (14)
|
NCWOW |
6:27.67 |
6:13.29 |
-14.38 |
| 14 |
Cornell, Meghan R (15)
|
NCHCAC |
5:52.59 |
6:15.65 |
23.06 |
| 15 |
Hawthorne, Morgan A (14)
|
NCWOW |
6:26.03 |
6:17.85 |
-8.18 |
| 16 |
McQueen, Elizabeth P (14)
|
NCWOW |
6:07.84 |
6:20.44 |
12.60 |
| 17 |
Davis, Mary R (13)
|
NCBAC |
6:24.85 |
6:20.48 |
-4.37 |
| 18 |
Witmer, Keener L (13)
|
NCNSEA |
6:37.04 |
6:21.14 |
-15.90 |
| 19 |
Munzenmaier, Sarah A (14)
|
NCHCAC |
6:02.20 |
6:21.83 |
19.63 |
| 20 |
Backman, Emmie N (13)
|
NCNSEA |
6:28.89 |
6:22.83 |
-6.06 |
| 21 |
Chambliss, Caroline J (13)
|
NCCFAC |
|
6:34.57 |
- |
| 22 |
King, Anna R (13)
|
NCCFAC |
|
6:44.47 |
- |
| 23 |
Reiniche, Katherine F (13)
|
NCWOW |
7:30.73 |
7:07.41 |
-23.32 |
| 24 |
Knudsen, Alexis P (15)
|
NCUN |
|
7:55.98 |
- |