| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rountree, Finley S (12)
|
NCWOW |
38.15 |
37.40 |
-0.75 |
| 2 |
Crane, Taylor M (11)
|
NCWOW |
37.71 |
37.51 |
-0.20 |
| 3 |
Weigand, Kelsey C (11)
|
NCWOW |
39.19 |
37.71 |
-1.48 |
| 4 |
Steele, Eliana C (12)
|
NCWOW |
41.49 |
38.08 |
-3.41 |
| 5 |
Seay, Caroline H (12)
|
NCWOW |
|
41.79 |
- |
| 6 |
Bollenbacher, Heidi F (11)
|
NCWOW |
41.31 |
42.68 |
1.37 |
| 7 |
Hughes, Chloe A (11)
|
NCWOW |
42.35 |
43.14 |
0.79 |
| 8 |
Norris, Chloe L (12)
|
NCWOW |
42.75 |
43.31 |
0.56 |
| 9 |
Seward, Emerson M (12)
|
NCWOW |
|
45.53 |
- |
| 10 |
Bartlett, Kyla E (11)
|
NCWOW |
46.84 |
46.04 |
-0.80 |
| 11 |
Mottram, Elynor G (11)
|
NCWOW |
50.70 |
48.44 |
-2.26 |
| 12 |
Keeler, Samantha B (12)
|
NCWOW |
55.35 |
50.22 |
-5.13 |
| 13 |
Sterner, Adalyn G (11)
|
NCWOW |
51.96 |
51.58 |
-0.38 |
| 14 |
Hoey, Parker J (11)
|
NCWOW |
|
52.28 |
- |
| 15 |
Santucci, Skylar (12)
|
NCWOW |
1:23.05 |
55.31 |
-27.74 |
|
Litton, Maegan Grace K (12)
|
NCWOW |
44.51 |
DQ |
- |