| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Winchester, Austin O (17)
|
NCWOW |
21.25 |
21.95 |
0.70 |
| 2 |
Furbay, Patrick D (16)
|
NCWOW |
21.64 |
22.59 |
0.95 |
| 3 |
Parker, William H (16)
|
UNAT |
21.65 |
23.00 |
1.35 |
| 4 |
Settlemyre, Jeffrey P (18)
|
NCWOW |
22.21 |
23.17 |
0.96 |
| 5 |
Page, Benjamin L (14)
|
NCWOW |
23.06 |
23.65 |
0.59 |
| 6 |
Tidball, Davis C (17)
|
UNAT |
|
23.66 |
- |
| 7 |
Davis, Matthew C (16)
|
NCWOW |
23.08 |
24.21 |
1.13 |
| 8 |
Butner, Mark L (16)
|
NCWOW |
23.39 |
24.71 |
1.32 |
| 9 |
Setzer, Sean T (14)
|
NCWOW |
23.68 |
25.29 |
1.61 |
| 10 |
Langtry, Ryan C (14)
|
NCWOW |
24.93 |
25.46 |
0.53 |
| 11 |
Post, Cian D (13)
|
NCWOW |
26.15 |
25.76 |
-0.39 |
| 12 |
Lyons, Frank E (16)
|
NCWOW |
25.69 |
26.01 |
0.32 |
| 13 |
Stuck, Evan J (14)
|
NCWOW |
26.37 |
26.46 |
0.09 |
| 14 |
Durham, William L (14)
|
NCWOW |
26.39 |
26.67 |
0.28 |
| 15 |
Hyldahl, Cooper C (16)
|
NCWOW |
25.04 |
26.92 |
1.88 |
| 16 |
Doyle, Kellan K (15)
|
NCWOW |
27.52 |
26.98 |
-0.54 |
| 17 |
Hughes, Jackson C (13)
|
NCWOW |
25.74 |
27.02 |
1.28 |
| 18 |
Eberhardt, Nathan L (14)
|
NCWOW |
28.14 |
28.53 |
0.39 |
| 19 |
Tillery, Wade H (13)
|
NCWOW |
30.55 |
30.47 |
-0.08 |
| 20 |
Mejouev, Maxim (13)
|
NCWOW |
30.30 |
31.32 |
1.02 |
|
Caravaglio, Matthew B (14)
|
NCWOW |
31.78 |
NS |
- |